Tropical Medicine

Article Infectious Diseases

Plasmodium infections and associated risk factors among parturients in Jawi district, northwest Ethiopia: a cross-sectional study

Zemenu Tamir, Abebe Animut, Sisay Dugassa, Mahlet Belachew, Adugna Abera, Aster Tsegaye, Berhanu Erko

Summary: The study finds a significant prevalence of asymptomatic Plasmodium infections among parturients in northwest Ethiopia. Risk factors for malaria during pregnancy include maternal illiteracy, primigravidity, lack of antenatal care follow-up, and history of symptomatic malaria during pregnancy. Promoting healthy pregnancies through ANC follow-up, strengthening malaria prevention and control practices, and screening asymptomatic pregnant women for malaria are suggested to reduce its burden during pregnancy.

MALARIA JOURNAL (2023)

Article Infectious Diseases

UPLC/ESI/MS profiling of red algae Galaxaura rugosa extracts and its activity against malaria mosquito vector, Anopheles pharoensis, with reference to Danio rerio and Daphnia magna as bioindicators

Mohamed A. M. El-Tabakh, Esraa A. Elhawary, Hossam M. Hwihy, Kareem F. Darweesh, Raafat M. Shaapan, Emad A. Ghazala, Mostafa M. Mokhtar, Hassan O. Waheeb, Deyaa E. M. Emam, Nader A. Bakr, Ahmed Z. I. Shehata

Summary: This study aims to investigate the activity of Galaxaura rugosa extracts against Anopheles pharoensis and non-target organisms, as well as to identify its chemical constituents using UPLC/ESI/MS.

MALARIA JOURNAL (2023)

Article Parasitology

Epidemiological, serological, and entomological aspects of visceral leishmaniasis (VL) in suspected new VL foci in Bangladesh

Debashis Ghosh, Md Utba Rashid, Soumik Kha Sagar, Md Rasel Uddin, Shomik Maruf, Prakash Ghosh, Rajashree Chowdhury, Md Abu Rahat, Md Nazmul Islam, M. M. Aktaruzzaman, Abu Nayeem Mohammad Sohel, Megha Raj Banjara, Axel Kroeger, Abraham Aseffa, Dinesh Mondal

Summary: The study aimed to explore the epidemiological, serological, and entomological aspects of visceral leishmaniasis (VL) in suspected new VL foci and assess the knowledge, attitude, and practices of the community living in the alleged new VL foci. The study found some areas with VL infection but no new active cases in the suspected new foci, highlighting the need for early detection and community awareness to control the spread of the disease.

ACTA TROPICA (2023)

Article Parasitology

Both C57BL/KsJ (H2d haplotype) and CB10-H2 (H2b haplotype) mice are highly susceptible to congenital toxoplasmosis

Loyane Bertagnolli Coutinho, Mario Cezar de Oliveira, Ester Cristina Borges Araujo, Flavia Batista Ferreira Franca, Marcos Paulo Oliveira Almeida, Yusmaris Cariaco, Paulo Czarnewski, Neide Maria Silva

Summary: Congenital toxoplasmosis can lead to adverse pregnancy outcomes. This study found that C57BL/KsJ mice were more susceptible to congenital toxoplasmosis compared to CB10-H2 mice, and there were differences in systemic cytokine levels between the two mouse lineages.

ACTA TROPICA (2023)

Article Parasitology

Classification and identification of mosquitoes in China based on rDNA 28S D5 region

Li Hu, Guodian Xiong, Yae Zhao, Rong Chai, Jiaxuan Xie, Yuyao Xiao, Yue Du, Juan Teng, Wanyu Zhang, Chenglin Guan

Summary: Accurate classification and identification of mosquitoes are crucial for preventing and controlling mosquito-borne diseases. This study examined mosquitoes from different cities in China and utilized morphological and molecular methods to identify and classify them. The results showed that the rDNA 28S D5 region can effectively distinguish different species of mosquitoes.

ACTA TROPICA (2023)

Article Parasitology

Proteomic profile of the venom of three dark-colored Tityus (Scorpiones: Buthidae) from the tropical rainforests of Costa Rica

Cecilia Diaz, Marcela Serna-Gonzalez, Arturo Chang-Castillo, Bruno Lomonte, Fabian Bonilla, Adriana Alfaro-Chinchilla, Felipe Triana, Mahmood Sasa

Summary: The objective of this study was to examine the differences in venom peptide sequences of Tityus species in Costa Rican rainforests compared to T. cf. asthenes from Colombia. The results showed that the venom peptides of dark-colored Tityus in Costa Rica are similar to those found in other T. (Atreus) species from the region. This suggests that the level of toxicity caused by Tityus in Costa Rica should be similar to that caused by members of the same subgenus in other geographical locations. The lack of epidemiological evidence of medical emergencies caused by envenoming by this scorpion group in Costa Rica may be more related to ecological and demographic factors.

ACTA TROPICA (2023)

Article Parasitology

Evaluation of isotype-based serology for diagnosis of Schistosoma mansoni infection in individuals living in endemic areas with low parasite burden

Fernanda do Carmo Magalha, Joao Marcelo Peixoto Moreira, Michelle Carvalho de Rezende, Vivian Favero, Carlos Graeff-Teixeira, Paulo Marcos Zech Coelho, Mariangela Carneiro, Stefan Michael Geiger, Deborah Negrao-Correa

Summary: Intestinal schistosomiasis is a chronic and debilitating disease that affects public health systems worldwide. Serum reactivity levels of different immunoglobulin isotypes can improve the diagnosis of infected individuals with low parasite loads.

ACTA TROPICA (2023)

Article Infectious Diseases

Can antibody conjugated nanomicelles alter the prospect of antibody targeted therapy against schistosomiasis mansoni?

Eglal I. Amer, Sonia R. Allam, Aceel Y. Hassan, Esmail M. El-Fakharany, Mona M. Agwa, Sherine N. Khattab, Eman Sheta, Marwa H. El-Faham

Summary: This study proposes a novel antibody-targeted nano-sized conjugated linoleic acid (CLA) delivery system for the treatment of Schistosoma mansoni infection and control of its transmission. The antibody-conjugated CLA nanomicelles showed significant schistosomicidal and anti-pathology activities against early and late stages of infection in a mouse model. The study highlights the potential of nanotechnology-based immunotherapy for parasitic infections and suggests that the immunodominant reactivity of the anti-SmI serum merits attention as a therapeutic candidate.

PLOS NEGLECTED TROPICAL DISEASES (2023)

Article Infectious Diseases

Public knowledge, belief, and preventive practices regarding dengue: Findings from a community-based survey in rural Bangladesh

Rajon Banik, Saiful Islam, Mahfuza Mubarak, Mahmudur Rahman, Hailay Abrha Gesesew, Paul R. Ward, Tajuddin Sikder

Summary: Dengue fever is a recurrent public health threat in rural Bangladesh, and there is a lack of knowledge and preventive practices among community people. This study assessed the knowledge, beliefs, and preventive practices regarding dengue among rural community people. The findings revealed a moderate level of knowledge and the need for expanded education outreach to increase public awareness and encourage preventive practices.

PLOS NEGLECTED TROPICAL DISEASES (2023)

Article Infectious Diseases

Mycobacterium ulcerans-Bordetella trematum chronic tropical cutaneous ulcer: A four-case series, Côte d'Ivoire

Bi Gore Oscar Tchan, Solange Kakou-Ngazoa, Sylveste Dizoe, Nassim Hammoudi, Ghiles Grine, Raymond Ruimy, Michel Drancourt, Roderick Hay

Summary: This study identified co-infection of M. ulcerans and other bacteria in patients with Buruli ulcer in Cote d'Ivoire, and revealed a specific co-pathogen B. trematum which counteracts the current treatment of Buruli ulcer in West Africa and Australia.

PLOS NEGLECTED TROPICAL DISEASES (2023)

Article Infectious Diseases

Differential cellular immune responses against Orientia tsutsugamushi Karp and Gilliam strains following acute infection in mice

Joseph D. Thiriot, Yuejin Liang, Casey Gonzales, Jiaren Sun, Xiaoying Yu, Lynn Soong

Summary: Scrub typhus, caused by Orientia tsutsugamushi (Ot), is a leading cause of febrile illness in endemic countries. Pulmonary involvement associated with vascular parasitism is common and can become life threatening. The antigenicity and genome content of Ot genotypes are connected to varied virulence and clinical outcomes. This study investigated the pulmonary immune responses of two prevalent bacterial strains (Karp and Gilliam) and identified potential biomarkers of disease severity.

PLOS NEGLECTED TROPICAL DISEASES (2023)

Article Infectious Diseases

The Chikungunya virus: A reemerging cause of acute febrile illness in the high jungle of northern Peru

Miguel Angel Aguilar-Luis, Hugh Watson, Yordi Tarazona-Castro, Lucinda Troyes-Rivera, Felipe Cabellos-Altamirano, Wilmer Silva-Caso, Ronald Aquino-Ortega, Hugo Carrillo-Ng, Victor Zavaleta-Gavidia, Juana del Valle-Mendoza

Summary: The study conducted in the high jungle of northern Peru reveals a significant prevalence of CHIKV infections among febrile patients. The findings underscore the importance of improving epidemiological surveillance and implementing reliable diagnostic techniques due to the nonspecific clinical symptoms of CHIKV infection.

PLOS NEGLECTED TROPICAL DISEASES (2023)

Article Parasitology

Tubulin as a potential molecular target for resveratrol in Giardia lamblia trophozoites, in vitro and in silico approaches

Jose Roberto Vargas-Villanueva, Filiberto Gutierrez-Gutierrez, Mariana Garza-Ontiveros, Sendar Daniel Nery-Flores, Lizeth Guadalupe Campos-Muzquiz, Dagoberto Vazquez-Obregon, Raul Rodriguez-Herrera, Lissethe Palomo-Ligas

Summary: Resveratrol has been found to inhibit the growth and adherence of Giardia and induce apoptosis-like cell death through tubulin alterations. Molecular docking analysis suggested that resveratrol binds to the interface heterodimer of Giardia tubulin, affecting microtubular dynamics. These findings suggest that resveratrol may be a potential drug for the treatment of giardiasis.

ACTA TROPICA (2023)

Article Parasitology

A novel sensitive hexaplex high-resolution melt assay for identification of five human Plasmodium species plus internal control

Suttipat Srisutham, Paweesuda Rattanakoch, Kaewkanha Kijprasong, Rungniran Sugaram, Nantanat Kantaratanakul, Theerarak Srinulgray, Arjen M. Dondorp, Mallika Imwong

Summary: A multiplex high-resolution melting (hexaplex PCR-HRM) assay was developed in this study for the simultaneous detection and differentiation of five human Plasmodium species, with the advantage of detecting the second minor parasite species.

ACTA TROPICA (2023)

Article Parasitology

Cellular mediators in human leishmaniasis: Critical determinants in parasite killing or disease progression

F. Divenuto, N. Marascio, A. Quirino, A. Giancotti, S. Filice, S. Gigliotti, M. P. Campolo, M. Campolo, G. S. Barreca, A. G. Lamberti, G. Castelli, F. Bruno, G. Matera

Summary: Data on cellular immunity mediators in the early phase of human leishmaniasis is limited and controversial. This study showed that different species of Leishmania can up-regulate various groups of cytokines and modulate NO release in different ways. These findings may contribute to the development of new therapeutics or vaccination strategies.

ACTA TROPICA (2023)

Review Parasitology

Leishmania species infection of bats: A systematic review

Nubia Nunes de Souza, Renata Luiz Ursine, Dardiane Santos Cruz, Emanuelle de Moura Santos Xavier, Lorena dos Reis Pereira Queiroz, Luiz Alberto Dolabela Falca, Walter Santos de Araujo, Celia Maria Ferreira Gontijo, Maria Norma Melo, Thallyta Maria Vieira

Summary: This study conducted a worldwide systematic review and found that bats may serve as hosts for Leishmania and likely play an important role in maintaining the parasite's life cycle. The results suggest that bats can be infected with a wide variety of Leishmania species.

ACTA TROPICA (2023)

Article Parasitology

Malabaricones from the fruit of Myristica cinnamomea King as potential agents against Acanthamoeba castellanii

Usman Ahmed, Yasodha Sivasothy, Khalid Mohammed Khan, Naveed Ahmed Khan, Siti Mariam Abdul Wahab, Khalijah Awang, Muhamad Aqmal Othman, Ayaz Anwar

Summary: This study found that the ethyl acetate extract of Myristica cinnamomea King fruit exhibited anti-amoebic activity against A. castellanii and identified Malabaricones B and C as potent compounds against A. castellanii. In addition, both the extract and the compounds inhibited the encystation and excystation of A. castellanii.

ACTA TROPICA (2023)

Article Parasitology

Echinococcus granulosus promotes bone resorption by increasing osteoclasts differentiation

Haohao Sun, Sibo Wang, Wenbo Tan, Ye Li, Qian Ren, Yaqing Liu, Yiping Huang, Chenhui Shi, Jing Li

Summary: Osseous cystic echinococcosis (CE) is a rare disease caused by Echinococcus granulosus, characterized by high morbidity, disability, and mortality. This study investigated the mechanism of bone destruction caused by osseous CE and provided insights for treating the disease.

ACTA TROPICA (2023)

Article Parasitology

First Argentine database for the accurate identification of Brucella to species level by MALDI-TOF MS

Celina Analia Celestino, Maria Florencia Rocca, Sandra Marcela Ayala, Lucia Irazu, Gabriela Ileana Escobar

Summary: This study established a local database of protein spectra for Brucella and validated its accuracy in strain identification. The results showed that MALDI-TOF mass spectrometry is a fast and reliable method for identifying different Brucella species, with the advantage of reducing exposure time to pathogenic microorganisms for laboratorians.

ACTA TROPICA (2023)

Article Parasitology

Diet of the sylvatic triatomine Mepraia spinolai: Association with Trypanosoma cruzi infection near human settlements

Catalina Sierra -Rosales, Esteban San Juan, Nicol Quiroga, Raul Araya-Donoso, Aldo Solari, Juana P. Correa, Antonella Bacigalupo, Carezza Botto-Mahan

Summary: The proximity between infectious disease vector populations and human settlements, as well as the infection prevalence of vector populations, can determine the infection risk. In this study, the diet of the Chilean endemic triatomine was characterized, and it was found that infected kissing bugs had human and human-associated animals in their diet. The proportion of human and human-associated vertebrates in the diet of infected bugs was negatively associated with the distance from human settlements.

ACTA TROPICA (2023)