Microscopy

Article Microscopy

Is it mathematically correct to fit AFM data (obtained on biological materials) to equations arising from Hertzian mechanics?

S. V. Kontomaris, A. Stylianou, A. Georgakopoulos, A. Malamou

Summary: This paper provides conclusive answers to the mathematical question regarding the fitting approach in testing soft biological samples and introduces a new tool, depth-dependent mechanical properties maps, for nanomechanical characterization of biological samples.

MICRON (2023)

Article Anatomy & Morphology

A first insight into haemocytes of Pinctada imbricata radiata: A morpho-functional characterization

Maria Pagano, Jacopo Fabrello, Cristiana Roberta Multisanti, Giorgia Zicarelli, Maria Ciscato, Francesco Boldrin, Salvatore Giacobbe, Valerio Matozzo, Caterina Faggio

Summary: The pearl oyster Pinctada imbricata radiata successfully colonized the Mediterranean Sea via the Suez Canal and showed the ability to adapt to various environmental conditions. This study revealed its potential as a model organism in environmental biomonitoring and highlighted the need for further research on its biological adaptations in invaded ecosystems, considering the potential threats it poses to native species and ecosystems.

MICROSCOPY RESEARCH AND TECHNIQUE (2023)

Article Materials Science, Multidisciplinary

Extending Estimating Hydrogen Content in Atom Probe Tomography Experiments Where H2 Molecule Formation Occurs

Martin S. Meier, Megan E. Jones, Peter J. Felfer, Michael P. Moody, Daniel Haley

Summary: This study investigated a new method for accurately estimating hydrogen content within specimen in atom probe tomography experiments. The research showed a highly correlated relationship between field evaporation rate and hydrogen content, leading to a new method for measuring hydrogen content. By combining extrapolation technique with continuous cycling of the evaporation rate during APT experiments, spatially resolved imaging of hydrogen concentrations could be achieved without the need for deuteration.

MICROSCOPY AND MICROANALYSIS (2022)

Article Anatomy & Morphology

Elucidating the structural, anticancer, and antibacterial traits of Punica granatum peel extracts-mediated Ag and Ag/GO nanocomposites

Emima J. Jeronsia, R. Ragu, Jerline A. Mary, Jerome S. Das

Summary: Ag nanoparticles and Ag/GO nanocomposites were successfully synthesized using peel extracts of Punica granatum, showing excellent antibacterial efficacy against both gram positive and gram negative bacterial strains, as well as dose dependent anticancer activity against human breast cancer cell line (MCF-7).

MICROSCOPY RESEARCH AND TECHNIQUE (2022)

Article Microscopy

Pore scale image analysis for petrophysical modelling

Arnab Kumar Pal, Siddharth Garia, K. Ravi, Archana M. Nair

Summary: This study demonstrates the applicability of image analysis in analyzing and defining reservoir rock properties. Digital image techniques reveal the micro and macro-pore types and their connectivity across multiple scales. The study shows that porosity and permeability can be accurately evaluated using digital image analysis and statistical equations can be developed to understand the relationship between these parameters. The study highlights the importance of thresholding in evaluating porosity using digital images. The developed methodology can accurately characterize reservoirs based on pore networks using high-resolution imaging techniques.

MICRON (2022)

Review Cell Biology

Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies

Cinzia Brenna, Carolina Simioni, Gabriele Varano, Ilaria Conti, Eva Costanzi, Mattia Melloni, Luca Maria Neri

Summary: Understanding the inner morphology of intact tissues is a significant challenge in modern biology. Optical tissue clearing (OTC) and 3D imaging have emerged as solutions for volumetric imaging and have been applied in clinical settings for more accurate diagnosis.

HISTOCHEMISTRY AND CELL BIOLOGY (2022)

Review Cell Biology

Three-dimensional SEM, TEM, and STEM for analysis of large-scale biological systems

Snjezana Radulovic, Sowmya Sunkara, Reinhard Rachel, Gerd Leitinger

Summary: A major aim in structural cell biology is to analyze intact cells in three dimensions, visualize subcellular structures, and even localize proteins at the best possible resolution in three dimensions. Recently developed electron microscopy tools offer great resolution in three dimensions, but the challenge is to investigate smaller volumes. Different approaches, including array tomography, batch tomography, and scanning transmission electron tomography, were presented at the Microscopy Conference in Vienna in 2021 to overcome this challenge and provide increased 3D information.

HISTOCHEMISTRY AND CELL BIOLOGY (2022)

Article Microscopy

Attention-guided multi-scale deep object detection framework for lymphocyte analysis in IHC histological images

Zunaira Rauf, Anabia Sohail, Saddam Hussain Khan, Asifullah Khan, Jeonghwan Gwak, Muhammad Maqbool

Summary: This article proposes a lymphocyte analysis framework based on a deep convolutional neural network, which can effectively detect lymphocytes in immunohistochemistry images. The framework demonstrates excellent generalization on multiple datasets and has the potential to be developed into a medical diagnostic tool.

MICROSCOPY (2023)

Article Anatomy & Morphology

The investigation of in vitro effects of farnesol at different cancer cell lines

Betul Yilmaz Ozturk, Narmin Feyzullazade, Ilknur Dag, Tayfun Sengel

Summary: Farnesol showed concentration-dependent effects on cancer cell lines, reducing proliferation at certain doses while inducing growth at higher concentrations. However, it did not suppress cellular growth in healthy cell lines. TEM analysis revealed autophagic and apoptotic findings in cancer cell lines treated with farnesol, while healthy cell lines exhibited normal findings. This study provides valuable information regarding farnesol's potential as an anti-cancer agent.

MICROSCOPY RESEARCH AND TECHNIQUE (2022)

Article Cell Biology

A higher dose of PEGylated gold nanoparticles reduces the accelerated blood clearance phenomenon effect and induces spleen B lymphocytes in albino mice

Ahmed M. Abu-Dief, Mosa Alsehli, Aziz Awaad

Summary: This study investigated the effect of different dose concentrations of PEGylated nanomaterials on the accelerated blood clearance (ABC) phenomenon. It showed that a higher dose concentration of PEG reduced the occurrence of ABC, and PEG-coated AuNPs mainly accumulated in the liver and spleen. Furthermore, the study found that the number of B lymphocytes in the spleen was affected by repeated doses of PEG-coated AuNPs. Therefore, dose optimization of PEG molecules is crucial for the development of effective nanomaterial-based drug delivery systems and to control the ABC phenomenon.

HISTOCHEMISTRY AND CELL BIOLOGY (2022)

Article Anatomy & Morphology

Morphological investigation of the gills of the dusky grouper Epinephelus marginatus (Lowe 1834) using gross anatomy and scanning electron microscopy

Mohamed A. M. Alsafy, Samir A. A. El-Gendy

Summary: The current study aimed to investigate the morphological characteristics of the gills of the dusky grouper through gross morphology, scanning electron microscopy, and morphometric analysis. The study concludes that the structure and features of the gills of the fish are adapted to the marine environment.

MICROSCOPY RESEARCH AND TECHNIQUE (2022)

Article Anatomy & Morphology

Adaptive investigation of the optical properties of polymer fibers from mixing noisy phase shifting microinterferograms using deep learning algorithms

Gehad Abo-Lila, Taha Sokkar, Eman Seisa, Emam Omar

Summary: This article proposes an adaptive denoising method for investigating the optical and structural features of polymeric fibers from noisy phase shifting microinterferograms, using convolution neural networks for denoising. The experimental results show that this method can accurately identify and remove different classes of noise, achieving good results for the tested fibers.

MICROSCOPY RESEARCH AND TECHNIQUE (2022)

Article Anatomy & Morphology

Characterization of the primo vascular system in rabbit vagina

Fatma El-Zahraa A. Mustafa, Enas A. Abdelhafez, Hanan H. Abd-Elhafeez

Summary: A primo node was observed on the lymph vessel of the vagina, surrounded by mesothelial cells providing positive immunoreactivity to NSE and VEGF. Different sized sinuses, floating cells, telocyte-like cell, and primo microcells were detected as the main constituents of the primo node, with migratory cells passing from the node to the lymph vessel.

MICROSCOPY RESEARCH AND TECHNIQUE (2022)

Article Materials Science, Multidisciplinary

Integrated Differential Phase Contrast (IDPC)-STEM Utilizing a Multi-Sector Detector for Imaging Thick Samples

Zhongbo Li, Johannes Biskupek, Ute Kaiser, Harald Rose

Summary: This study evaluates the potential of integrated differential phase contrast (IDPC) imaging for thick samples. By varying the focus and using a multi-sector detector, the anisotropy of the contrast transfer function (CTF) can be eliminated, resulting in improved image contrast and resolution.

MICROSCOPY AND MICROANALYSIS (2022)

Article Cell Biology

Activation of RIPK2-mediated NOD1 signaling promotes proliferation and invasion of ovarian cancer cells via NF-κB pathway

WenJuan Zhang, YanYan Wang

Summary: This study revealed that NOD1 promotes ovarian cancer progression by activating the NF-κB signaling pathway, predominantly through binding to RIPK2. The findings suggest a new therapeutic strategy for the treatment of ovarian cancer.

HISTOCHEMISTRY AND CELL BIOLOGY (2022)

Article Microscopy

Sub-Sampled Imaging for STEM: Maximising Image Speed, Resolution and Precision Through Reconstruction Parameter Refinement

Daniel Nicholls, Jack Wells, Andrew Stevens, Yalin Zheng, Jony Castagna, Nigel D. Browning

Summary: Sub-sampling during image acquisition in STEM can increase the acquisition speed and control the dose effectively. Optimizing control parameters can improve the resolution, precision, and sensitivity of inpainted images. When combined with hardware for rapidly sub-sampling images in STEM, this approach has significant implications for imaging beam-sensitive materials and dynamic processes.

ULTRAMICROSCOPY (2022)

Article Materials Science, Multidisciplinary

Real-Time Integration Center of Mass (riCOM) Reconstruction for 4D STEM

Chu-Ping Yu, Thomas Friedrich, Daen Jannis, Sandra Van Aert, Johan Verbeeck

Summary: This paper proposes a real-time image reconstruction method for scanning transmission electron microscopy (STEM) that can update the resulting image without storing any intermediate diffraction patterns. The method produces high-quality reconstructions with good noise robustness and low computational requirements. An efficient open source implementation is also provided.

MICROSCOPY AND MICROANALYSIS (2022)

Article Anatomy & Morphology

Effect of green synthesized ZnO nanoparticles using Paspalum scrobiculatum grains extract in biological applications

K. Velsankar, G. Parvathy, S. Mohandoss, Sakkarapani Sudhahar

Summary: This report presents the biosynthesis of ZnO nanoparticles using Paspalum scrobiculatum grains extract and explores their various biological activities and potential applications as an efficient material.

MICROSCOPY RESEARCH AND TECHNIQUE (2022)

Article Anatomy & Morphology

Pollen morphology and its implication in the taxonomy of some selected taxa of the bi and tri-ovulate Euphorbiaceae of the Hainan Island by using multiple microscopic techniques

Saraj Bahadur, Sehrish Taj, Wenxing Long, Mushtaq Ahmad

Summary: This study documented the pollen morphology of Euphorbiaceae taxa collected from different regions of Hainan Island, China, and found that pollen traits have relevance in the taxonomy and phylogenetic of the family.

MICROSCOPY RESEARCH AND TECHNIQUE (2022)

Article Anatomy & Morphology

Biosynthesis of silver nanoparticles using endophytic Fusarium oxysporum strain NFW16 and their in vitro antibacterial potential

Nikhat Ilahi, Abdul Haleem, Sajid Iqbal, Nighat Fatima, Wasim Sajjad, Abubakar Sideeq, Safia Ahmed

Summary: Nanotechnology has provided a platform for producing functionalized and biocompatible silver nanoparticles (AgNPs) with promising applications. The AgNPs synthesized in this study showed strong antibacterial potential against multiple drug-resistant bacteria, and demonstrated synergistic effects with various antibiotics. Additionally, impregnated cotton and bandage with AgNPs displayed in vitro antibacterial activity against pathogenic bacteria.

MICROSCOPY RESEARCH AND TECHNIQUE (2022)