Article
Microscopy
Amel Shamseldeen Ali Alhassan, Siyuan Zhang, Benjamin Berkels
Summary: In this study, a novel method for automatic motif extraction in crystalline images based on a variational approach involving the unit cell projection operator is proposed and demonstrated. The method involves a multi-stage algorithm to determine the primitive unit cell and extract the motif image and atom positions. The method was tested on various synthetic and experimental images and proved to be effective.
Article
Microscopy
T. Kh. Kumachova, A. V. Babosha, A. S. Ryabchenko, A. S. Voronkov
Summary: This study investigated the micromorphology, histochemistry, and fluorescence characteristics of colleters on leaf structures of Mespilus germanica L. Colleters were found on the tips of leaf blades and stipules, and less frequently on the petioles. They were multicellular secretory structures and their secretion process occurred during the active growth of leaf structures. Polysaccharides, phenolic substances, and lipids were detected in the colleters. The fluorescence spectrum showed a peak at 671-672 nm upon excitation at 405 and 473 nm.
Article
Microscopy
Roberto Conconi, Gennaro Ventruti, Fernando Nieto, Giancarlo Capitani
Summary: This study compares quantification methods for energy dispersive X-ray microanalysis in transmission electron microscopy. The Cliff-Lorimer approximation and absorption correction method are found to provide better results compared to the standardless method. While CLA and ACM perform similarly when absorption can be considered negligible, ACM gives superior results for larger mass thickness. Caution is advised when deriving k-factors indirectly from minerals with different structures/chemistry, suggesting the need for separate k-factor data sets for accurate EDS quantification of major mineral classes. Element diffusion and channelling effects may complicate the analysis, especially in anisotropic minerals like phyllosilicates.
Article
Microscopy
Christine Moriscot, Guy Schoehn, Dominique Housset
Summary: Electron diffraction is an efficient technique for solving the structure of small organic molecules and model proteins. However, the major challenge when applying this technique to protein samples is to prepare nano-crystals of the appropriate size and deposit them onto a grid with minimal solvent. In this study, a new approach using cryo-sectioning after high pressure freezing was presented, allowing the preservation of protein structures at high resolution and serving as a new sample preparation technique for 3D electron diffraction experiments.
Article
Microscopy
Nicolas A. Rivas Rivas, Alba Garzon Manjon, Miquel Vega-Paredes, Se-Ho Kim, Baptiste Gault, Hosun Jun, Chanwon Jung, Viktoriya Berova, Katharina Hengge, Tilman Jurzinsky, Christina Scheu
Summary: Ru@Pt core shell nanoparticles exhibit optimal catalytic properties for anodic oxidation of H2, leading to reduced Pt loading and improved stability in hydrogen fuel cells by preferential CO oxidation. However, the presence of residual Cl and Na in the Ru nanoparticles synthesized using RuCl3·H2O precursor has not been extensively studied. Therefore, the combination of correlative atom probe tomography (APT) and scanning transmission electron microscopy ((S)TEM) was employed to fully characterize the structure and chemical makeup of Ru nanoparticles due to the limitations of traditional characterization methods.
Article
Microscopy
Bowen Zhong, Zhan Liao, Xiaopeng Hao, Junjie Hu, Lining Sun
Summary: This paper proposes a dynamic parallel image acquisition method to greatly improve the speed of image acquisition without compromising image clarity. It also designs a variable speed motion curve to ensure the quality of captured images. The experimental results demonstrate significant improvement in acquisition speed compared to traditional scanning mode.
Article
Microscopy
Christoph Hofer, Timothy J. Pennycook
Summary: Electron ptychography is an effective and sensitive technique for phase imaging. However, quantifying the ptychographic phases of atomic sites remains a challenge. This article presents a new method that overcomes this challenge, especially for 2D materials, and is robust to experimental parameters such as noise and sample tilt.
Article
Microscopy
Abeer A. Mahmoud, Maha Zayed Mohamed, Ebtehal Z. Hassen
Summary: Potassium bromate has degenerative effects on neurons in the cerebral cortex, while Urtica dioica provides neuroprotective effects through its antioxidant properties.
ULTRASTRUCTURAL PATHOLOGY
(2023)
Article
Anatomy & Morphology
Muhammad Faran Akhtar, Muhammad Irshad, Shaukat Ali, Muhammad Summer, Saima Gulrukh, Muhammad Irfan
Summary: Recent developments in the green synthesis of metallic nanoparticles using phytoconstituents have attracted global scientific attention. This study successfully synthesized silver nanoparticles using Punica granatum and Plectranthus rugosus plant extracts, and characterized their properties. The findings suggest that the synthesized silver nanoparticles under specific conditions have good potential for biomedical applications.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Review
Cell Biology
Thangarasu Rajakumar, Pachaiappan Pugalendhi
Summary: Cancer growth is a complex process involving genetic and epigenetic modifications. Chemoprevention, especially using naturally occurring phytochemicals like allyl isothiocyanate (AITC), has gained popularity due to its high bioavailability, safety, and efficacy. AITC has shown significant anticancer activity by selectively modulating various cell signaling pathways related to oxidative stress, inflammation, cell proliferation, apoptosis, angiogenesis, invasion, and metastasis.
HISTOCHEMISTRY AND CELL BIOLOGY
(2023)
Article
Anatomy & Morphology
Yasmeen A. S. Hameed, Nasser A. Alamrani, Sahar Sallam, Saham F. Ibarhiam, Albandary Almahri, Ali Q. Alorabi, Nashwa M. El-Metwaly
Summary: Smart clothing refers to textiles that can sense external stimuli by changing their physical properties. In this study, a novel method was used to apply a luminous and hydrophobic composite coating onto cellulose-based materials. The LSAO-finished viscose showed efficient antimicrobial, UV-protective, and superhydrophobic activities, and exhibited reversible photochromism under UV light and in the dark.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Letter
Biology
Yasuaki Tatsumi, Hisao Hayash, Koichi Kato
MEDICAL MOLECULAR MORPHOLOGY
(2023)
Article
Microscopy
Nataliya Bgatova, Sergey Savchenko, Alexei Lamanov, Iuliia Taskaeva, Boris Ayzikovich, Valentina Gritcinger, Andrey Letyagin, Maksim Korolev
Summary: This study examined the ultrastructural organization of endothelial cells in myocardial capillaries of COVID-19 patients. Electron microscopy revealed the presence of abundant rough endoplasmic reticulum, Golgi complex, and vesicles with virions and zippered endoplasmic reticulum in the endothelial cells. These findings suggest that COVID-19 can lead to remodeling of intracellular membranes and the formation of virus-induced structures critical for viral replication and assembly in myocardial endothelial cells. The data could provide insights into the development of endothelial dysfunction in COVID-19 patients and potential targets for drug therapy.
ULTRASTRUCTURAL PATHOLOGY
(2023)
Article
Microscopy
Peter J. O'Toole, Joanne L. Marrison
Summary: This article outlines the Bioscience Technology Facility (BTF) at the University of York as a prime example of the Full Cost Recovery model. It emphasizes that each facility operates differently and is subject to various regulations at different levels. The BTF model is able to fully recover its operating costs within an academic environment and has received institutional and funders support.
JOURNAL OF MICROSCOPY
(2023)
Article
Materials Science, Multidisciplinary
Ahmed M. Rashwan, Mohamed A. M. Alsafy, Samir A. A. El-Gendy, Ahmed A. El-Mansi, Samar M. Ez Elarab
Summary: This study investigated the expression and localization of telocytes and keratocytes in buffalo cornea using various microscopic techniques. The results suggest that telocytes and keratocytes play important roles in corneal wound healing, transparency maintenance, and homeostasis. The co-expression of certain markers indicates the potential presence of mesenchymal cells.
MICROSCOPY AND MICROANALYSIS
(2023)
Article
Materials Science, Multidisciplinary
Karen Salazar, Ademaria Novais, Jose Lino-Neto, Jose Eduardo Serrao
Summary: We investigated the reproductive tracts of Gyretes sp. using light and transmission electron microscopies. The male has testes and accessory glands, while the female has ovaries, fertilization duct, and an accessory gland. These findings provide important information for understanding the reproductive biology and taxonomy of Gyretes.
MICROSCOPY AND MICROANALYSIS
(2023)
Article
Materials Science, Multidisciplinary
Stanislav Polzer, Sarah Thompson, Swathi Vittalbabu, Arzu Ulu, David Carter, Tara Nordgren, Mona Eskandari
Summary: Knowledge of soft tissue fiber structure is necessary for accurate characterization and modeling of their mechanical response. This study develops an automatic algorithm to simultaneously estimate fiber global orientation, abundance, and waviness in an investigated image.
MICROSCOPY AND MICROANALYSIS
(2023)
Article
Anatomy & Morphology
Saher Fadl, Abdelmohaimen M. M. Saleh, Ahmed Abou-Elmagd, Fatma M. Abdel-maksoud
Summary: The present study investigated the histological, ultrastructural, and immunohistochemical findings of the adrenal gland in Japanese quail during the prehatching phase. The results showed significant morphological changes in the adrenal gland during prenatal life. Studying the prenatal development of the adrenal gland in birds is important for advancing our understanding of this critical organ and its functions.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Anatomy & Morphology
Subok Kim, Sanghun Jang, Onseok Lee
Summary: This study proposes a 3D assessment technique using synchrotron radiation x-ray microcomputed tomography to analyze the functionality and curvature damage of a single fiber in a stroke-induced muscle. The results show that the curvature damage in the impaired muscle progresses after the stroke, and the SF volume is significantly reduced. This study has important implications for the field of rehabilitation therapy.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Anatomy & Morphology
Subok Kim, Sanghun Jang, Onseok Lee
Summary: This study aimed to objectively diagnose osteoporosis (OP) by visualizing micro-damage caused by OP using synchrotron radiation-based mu CT (SR-mu CT). The results showed that the OVX group had smaller cortical bone volume and thickness, as well as lower trabecular bone volume/total volume compared to the SHAM group. In addition, the SHAM group had larger canal volume and lacuna volume than the OVX group.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
