Article
Anatomy & Morphology
Farhat Ullah, Mushtaq Ahmad, Muhammad Zafar, Bushra Parveen, Shomaila Ashfaq, Saraj Bahadur, Qura-tul-ain Safdar, Luqman Bin Safdar, Fakhre Alam, Muhammad Luqman
Summary: The study on pollen micromorphology of selected taxa from the subfamily Caesalpiniaceae in different geographical regions of Pakistan revealed significant variations in equatorial shape, surface ornamentation, tectum, polar diameter, equatorial diameter, and exine thickness, highlighting the importance of pollen traits in species identification and classification.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Anatomy & Morphology
Yuefei Lin, Yong Diao, Yongzhao Du, Jianguang Zhang, Ling Li, Peizhong Liu
Summary: The proposed method for cell counting, which combines Otsu and watershed segmentation methods, can achieve automatic counting with high accuracy. Experimental results show that the method is fast and accurate in cell counting.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Anatomy & Morphology
Rozina, Mushtaq Ahmad, Muhammad Zafar, Zainab Yousaf, Sher Aman Ullah, Shazia Sultana, Farhana Bibi
Summary: In the present era, environmental issues and energy crises are driving attention towards renewable energy sources like biodiesel. This study focused on investigating the micromorphological characteristics of non-edible seed oils for biodiesel production using SEM. Results revealed variations in seed size, shape, color, oil content, and FFA content. SEM analysis showed distinctive surface features on seeds, providing useful information for accurate identification and classification by researchers.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Cell Biology
Maria Munhoz Evangelinellis, Roberta Figueiroa Souza, Cristina Eusebio Mendes, Patricia Castelucci
Summary: Inflammatory bowel diseases (IBDs), such as ulcerative colitis and Crohn's disease, are chronic conditions affecting the gastrointestinal tract. Research has shown that the P2X7 receptor antagonist Brilliant Blue G (BBG) can restore enteric neurons, with potential therapeutic implications. In an experimental rat model of ulcerative colitis induced by TNBS, BBG was found to improve disease activity index, histological tissue integrity, neuronal density, and the profile of specific enteric neurons. These results suggest that BBG may be effective in improving ulcerative colitis and that the P2X7 receptor could be a therapeutic target.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Microscopy
S. V. Kontomaris, A. Malamou, A. Stylianou
Summary: This review discusses the procedures for accurately processing force-indentation curves in biological sample experiments, including the avoidance methods for common errors such as the shape of the AFM tip, the range of data of the sample's approximate linear elastic response, the sample's viscoelasticity, the sample's shape, and the substrate effects.
Article
Cell Biology
Maria de los Angeles Castillo-Rodriguez, Stefan Gingele, Lara-Jasmin Schroeder, Thiemo Moellenkamp, Martin Stangel, Thomas Skripuletz, Viktoria Gudi
Summary: The loss of myelin, degeneration of axons, and impaired remyelination contribute to the progressive disease in multiple sclerosis patients. Astrocytes play a vital role in these processes. Our study identified GFAP, vimentin, LCN2, and ALDH1L1 as reliable markers for activated astrocytes during cuprizone-induced de- and remyelination.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Microscopy
R. F. Egerton, M. Watanabe
Summary: This article reviews the practical factors that determine the spatial resolution of TEM and STEM, and discusses the advantages of representing resolution in terms of a point-spread function. The article provides the point-spread functions for various resolution-limiting factors and compares beam broadening in different materials. Additionally, the importance of dose-limited resolution (DLR) for beam-sensitive specimens is emphasized, along with efforts to overcome the fundamental resolution limits.
Article
Anatomy & Morphology
Shikhgasan Ramazanov, Dinara Sobola, Stefan Talu, Farid Orudzev, Ali Arman, Pavel Kaspar, Rashid Dallaev, Guseyn Ramazanov
Summary: Thin films of bismuth and iron oxides were successfully deposited on a flexible Kapton substrate using ALD technique, exhibiting uniform distribution of elements, electrical polarization, and ferromagnetic response. The functionalization of Kapton surface with bismuth and iron oxides allows for the adaptation of Bi-Fe-O system to flexible electronics.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Materials Science, Multidisciplinary
Vitor Vieira Rielli, Felix Theska, Sophie Primig
Summary: PFIB is a novel nanofabrication technique suitable for site-specific atom probe sample preparation; TKD is a method that facilitates phase identification and crystallographic measurements in electron transparent samples; Using PFIB and TKD for atom probe tomography tip preparation is highly effective for challenging material interfaces.
MICROSCOPY AND MICROANALYSIS
(2022)
Review
Materials Science, Multidisciplinary
Manale Noun, Rayane Akoumeh, Imane Abbas
Summary: Mass spectrometry imaging (MSI) is a powerful technique that can reveal the spatial distribution of atomic and molecular ions on the surfaces of biological samples. MALDI-TOF and TOF-SIMS, two commonly used MSI techniques, have witnessed significant advancements in studying cells and tissue sections due to their high resolution, sensitivity, precision, and chemical specificity. This article briefly discusses the principles and sampling methods of MALDI-TOF and TOF-SIMS, while highlighting their importance as diagnostic tools and robust analytical techniques in the medicinal, pharmaceutical, and toxicology fields through representative published studies.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Microscopy
Kunwar Muhammed Saaim, Saima Khan Afridi, Maryam Nisar, Saiful Islam
Summary: Computer scientists have been working on models to save laboratory expenses and time for the scientific community. This study focuses on finding the best segmentation model for nanoparticles in microscopy images and examines its robustness and generalizability across different microscopy parameters.
Article
Anatomy & Morphology
Taj Ur Rahman, Hammad Khan, Wajiha Liaqat, Muhammad Aurang Zeb
Summary: The phytochemical tests of Ricinus communis L methanolic extract revealed the presence of certain compounds, and gold nanoparticles were successfully synthesized from the extract. Antibacterial tests against different strains showed inhibitory effects on specific bacteria by the extract and nanoparticles.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Microscopy
A. Karali, A. P. Kao, R. Meeson, M. Roldo, G. W. Blunn, G. Tozzi
Summary: This study investigated the strain distribution and load-bearing capacity in regenerating fractures using in situ XCT mechanics and DVC. The results showed significant variation and inhomogeneity in the 3D strain distribution in the regenerating tissues of the fracture gap, which was associated with the presence of unmineralised tissue observed in histological images.
JOURNAL OF MICROSCOPY
(2022)
Article
Materials Science, Multidisciplinary
Justyna A. Niestrawska, Anna Pukaluk, Anju R. Babu, Gerhard A. Holzapfel
Summary: The micro-structural changes of aortic collagen during disease development are important for understanding collagen reorganization. Second-harmonic generation microscopy is used to study human aortic collagen in healthy and diseased states. Methods are being developed to determine the waviness, diameter, orientation, and dispersion of collagen fibers and bundles.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Microscopy
S. I. Shakil, A. S. Zoeram, M. Avateffazeli, M. Roscher, H. Pirgazi, B. Shalchi-Amirkhiz, B. Poorganji, M. Mohammadi, M. Haghshenas
Summary: A dual-stage indentation test was conducted to assess the time-dependent plastic deformation of cast and additive manufactured Al-Cu-Mg-Ag-TiB2 alloys. The microstructure of the samples and its correlation with creep properties were studied using different microscopy techniques. The results showed that the microstructure and precipitation density had a significant impact on the indentation-derived properties and the creep resistance. Dislocation creep was identified as the main controlling mechanism of creep deformation.
Article
Materials Science, Multidisciplinary
Colum M. O'Leary, Benedikt Haas, Christoph T. Koch, Peter D. Nellist, Lewys Jones
Summary: The article presents an extension of existing STEM distortion correction techniques for the treatment of 4D data series, demonstrating improvements in spatial fidelity, signal-to-noise ratio (SNR), phase precision, and spatial resolution using electron ptychography and electric-field mapping as model cases.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Anatomy & Morphology
Arpita Dey, Sundarapandian Somaiah
Summary: This study reported a green chemistry-based approach for synthesizing zinc oxide nanoparticles using plant extract to reduce metal ions, focusing on minimizing toxic reactants and byproducts. Physicochemical characterizations and mechanisms of action were presented, showing excellent free radical scavenging activity of the prepared nanoparticles but no antibacterial activity against pathogenic bacteria.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Microscopy
Silvia Castro Coelho, Philomene Benaut, Sebastien Laget, Berta N. Estevinho, Fernando Rocha
Summary: Electrohydrodynamic techniques were used to optimize zein microstructures for encapsulation purposes, resulting in compact electrosprayed microbeads and uniform smooth electrospun fibers. The study investigated the effects of zein polymer properties, flow rate, applied voltage, and distance tip-collector on the morphology of the structures. These zein microstructures have the potential to encapsulate bioactive ingredients for various applications in functional food, nutraceutical, and medical fields.
Article
Anatomy & Morphology
Kanwal Shahzad, Muhammad Zafar, Amir Muhammad Khan, Tariq Mahmood, Qamar Abbas, Fethi Ahmet Ozdemir, Mushtaq Ahmad, Shazia Sultana
Summary: In this study, 22 herbaceous plant specimens were examined using a light microscope to investigate leaf epidermal anatomy. Significant variations in qualitative and quantitative features were observed, providing valuable insights for taxonomists. The foliar epidermal anatomy of herbaceous flora can serve as baseline data for demarcation and limitation of associated taxa.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Microscopy
Niels Cautaerts, Phillip Crout, Hakon W. Anes, Eric Prestat, Jiwon Jeong, Gerhard Dehm, Christian H. Liebscher
Summary: This work presents the new template matching capabilities implemented in Pyxem, an open source Python library for analyzing 4D-STEM data. The method derives local crystal orientations by comparing simulated diffraction patterns to experimental patterns. It is computationally demanding and utilizes multiple CPU cores or GPUs for efficiency and scalability. The implementation is designed to support custom and reproducible workflows and can handle pixelated detectors of different sizes and formats. The paper details the method's implementation and validation, with examples of orientation mapping in nanocrystalline materials and precipitates. The combination of speed and flexibility enables automated parameter studies and real-time on-line orientation mapping within the TEM.
