Microscopy

Article Anatomy & Morphology

A novel image processing procedure for the quantitative evaluation of dental enamel prism arrangement

Mate Hegedus, Viktoria K. Kis, Noemi Rozsa, Zsolt Kovacs

Summary: A novel image processing method based on scanning electron microscopy images and ellipse fitting is developed to quantitatively characterize the shape and orientation of enamel prisms. Color coded orientation maps provide quick insight into the microstructure of enamel and allow for statistical analysis of prism arrangement. This method contributes to the quantitative investigation of prism arrangement in dentistry research and evolution biology.

MICROSCOPY RESEARCH AND TECHNIQUE (2023)

Article Microscopy

The dam that fly ash built

Amy Montgomery, Mahipal Kasaniya, Pengfei Zhao, Michael Thomas, Karl Peterson

Summary: Hungry Horse Dam stands out as the first dam constructed using coal combustion fly ash, directly connected to historical periods of massive adoption of coal as fuel for power plants and threats to fresh water supply and glacier retreat due to global warming.

JOURNAL OF MICROSCOPY (2023)

Article Anatomy & Morphology

Microstructural and histochemical analysis of shoots and cones of Juniperus seravschanica (Cupressaceae)

Farkhondeh Rezanejad, Farzad Ganjalikhani Hakemi

Summary: Juniper species contain abundant compounds that have various applications and protect the plants from stress. This study investigated the cytochemical and histochemical traits of Juniperus seravschanica, revealing the composition and distribution of different compounds in leaves, stems, and female cones. The high levels of various compounds in the female cones contribute to their resistance and medicinal role.

MICROSCOPY RESEARCH AND TECHNIQUE (2023)

Article Anatomy & Morphology

A detailed anatomical approach to taxonomically problematic endemic Seseli L. (Apiaceae) taxa in Turkiye

Dudu Ozlem Mavi Idman

Summary: This study compares the vegetative anatomical features of different Apiaceae species in Turkey, highlighting the importance of anatomy in taxonomy and providing valuable information for future research.

MICROSCOPY RESEARCH AND TECHNIQUE (2023)

Article Biology

Transmission electron microscopic study of the surface layer of surgical resected disc specimens in human temporomandibular joint

Hiroaki Yoshida, Hiroki Ishikawa, Akio Himejima, Hayato Ikeda, Mitsuru Tani, Ryoji Taniguchi, Tomio Iseki, Yutaka Tsutsumi

Summary: This study investigated the characteristics of the surface layer of surgical resected disc specimens in human temporomandibular joint osteoarthritis cases using transmission electron microscopy. The findings showed that the surface was covered with fibroblastic and histiocytoid cells, collagen fiber bundles and collagenous matrix were exposed, and fibrinous dense material was observed. Collagen bundles were rich around capillary vessels, and synovial surface cells exhibited features of activated macrophages with vacuole formation.

MEDICAL MOLECULAR MORPHOLOGY (2023)

Review Anatomy & Morphology

Atomic force microscopy in disease-related studies: Exploring tissue and cell mechanics

Shuaiyuan Liu, Yibo Han, Lingwen Kong, Guixue Wang, Zhiyi Ye

Summary: Atomic force microscopy shows promise as a clinical device to diagnose and treat challenging diseases by examining the surface morphology and assessing tissue and cellular mechanics of biological specimens in a physiological setting.

MICROSCOPY RESEARCH AND TECHNIQUE (2023)

Article Materials Science, Multidisciplinary

Comparison of Laboratory Diffraction Contrast Tomography and Electron Backscatter Diffraction Results: Application to Naturally Occurring Chromites

Xiao Chen, Belinda Godel, Michael Verrall

Summary: This study demonstrates the significant contribution of LabDCT in understanding complex rock materials from an earth science perspective, particularly in characterizing mineral texture and crystallography in 3D.

MICROSCOPY AND MICROANALYSIS (2023)

Article Materials Science, Multidisciplinary

High Temperature-Induced m6A Epigenetic Changes Affect Early Porcine Embryonic Developmental Competence in Pigs

Ming-Hong Sun, Wen-Jie Jiang, Xiao-Han Li, Song-Hee Lee, Geun Heo, Dongjie Zhou, Jing Guo, Xiang-Shun Cui

Summary: This study revealed the impact of high temperature exposure on early porcine embryonic development. It was found that high temperature can alter m(6)A modification levels, which in turn affects blastocyst formation.

MICROSCOPY AND MICROANALYSIS (2023)

Article Materials Science, Multidisciplinary

Deep Learning Approach for High-accuracy Electron Counting of Monolithic Active Pixel Sensor-type Direct Electron Detectors at Increased Electron Dose

Jingrui Wei, Kalani Moore, Benjamin Bammes, Barnaby D. A. Levin, Nicholas Hagopian, Ryan Jacobs, Dane Morgan, Paul M. Voyles

Summary: In this study, a supervised deep learning approach was proposed to recognize single electron events at varying doses and voltages. The DL approach showed high accuracy and better performance at higher doses compared to conventional counting methods.

MICROSCOPY AND MICROANALYSIS (2023)

Article Microscopy

Comparative effects of chemical and green zinc oxide nanoparticles in caprine testis: ultrastructural and steroidogenic enzyme analysis

Sneha Bareja, Rajnesh Kumar Sharma

Summary: Advancements in nanotechnology have opened up possibilities in various sectors, but also introduced toxic risks of engineered nanoparticles on living organisms. This study found that chemically synthesized zinc oxide nanoparticles are more toxic than green zinc oxide nanoparticles, adversely affecting the testicular tissue of goats.

ULTRASTRUCTURAL PATHOLOGY (2023)

Article Anatomy & Morphology

Morphology of the small and large intestines of the six-banded armadillo (Euphractus sexcinctus) Linnaeus, 1758

Clarisse Maria Barbosa Fonseca, Ingrid dos Santos Farias, Joao Victor Silva Araujo, Rita de Cassia Viana, Andrezza Braga Soares da Silva, Maria Michele Araujo de Sousa Cavalcante, Airton Mendes Conde

Summary: The objective of this study was to examine the morphology and histological features of the small and large intestines of the six-banded armadillo (E. sexcinctus). The results showed that the small intestine has three parts and the typical four layers of tubular organs, while the large intestine has similar histological subdivisions as the small intestine but with some specializations.

MICROSCOPY RESEARCH AND TECHNIQUE (2023)

Article Anatomy & Morphology

Prenatal developmental sequences of the esophageal epithelium in the New Zealand white rabbits: Light and electron microscopic analysis

Wafaa Gaber, Fatma Khalil, Dalia Mohamedien

Summary: This study investigated the cellular events in the morphogenesis of rabbit esophageal epithelium during development, and found that the type and number of cells in the epithelium changed during the developmental process, with mucous cells and dark cells playing important roles in the later stages of development.

MICROSCOPY RESEARCH AND TECHNIQUE (2023)

Review Anatomy & Morphology

Harnessing atomic force microscopy-based single-cell analysis to advance physical oncology

Mi Li

Summary: Single-cell analysis is a promising field in life sciences, allowing the exploration of fundamental laws of physiological and pathological processes. Atomic force microscopy (AFM) is a powerful tool capable of non-destructive imaging and quantitative measurement of structural and mechanical properties of single living cells. AFM has been widely used in cancer research, providing unique insights into tumor pathogenesis from a biomechanical perspective.

MICROSCOPY RESEARCH AND TECHNIQUE (2023)

Article Anatomy & Morphology

Russula brunneoaurantiaca, a novel taxon of Russula subg. Crassotunicata from West Bengal, India, with morpho-molecular analysis and scanning electron microscopy

Niranjan Roy, Jeswani Beypih, Bhaben Tanti, Arun Kumar Dutta

Summary: This paper describes a new species of the Russula genus with detailed morphological and molecular sequence data, along with comparisons to closely related species.

MICROSCOPY RESEARCH AND TECHNIQUE (2023)

Article Anatomy & Morphology

Enhancing SEM positioning precision with a LEGO®-based sample fitting system

Piero Antonio Zecca, Andrea Brambilla, Marcella Reguzzoni, Marina Protasoni, Mario Raspanti

Summary: This study developed a LEGO-based sample positioning system for SEM analysis, which consistently identified and aligned features, maintained accurate positioning, and provided repeatable and reliable results. The system demonstrated high precision and accuracy in sample repositioning, with reliable replication of results. Further research is needed to optimize the system's design and evaluate its performance in different SEM applications.

MICROSCOPY RESEARCH AND TECHNIQUE (2023)

Article Microscopy

Scanning capacitance microscopy of GaN-based high electron mobility transistor structures: A practical guide

Chen Chen, Saptarsi Ghosh, Francesca Adams, Menno J. Kappers, David J. Wallis, Rachel A. Oliver

Summary: The researchers optimize the application of scanning capacitance microscopy in GaN-based HEMT structures through experimental setup and imaging conditions. They find that a sharp probe coated with conductive doped diamond, a large spring constant, and positioning close to the edge and Ohmic contact of the sample can improve contrast. In addition, applying a dc bias in addition to an ac bias is more effective in depleting the 2DEG in the AlGaN/GaN heterostructure. The selected ac bias has no significant impact on contrast.

ULTRAMICROSCOPY (2023)

Article Microscopy

Morphology measurements by AFM tapping without causing surface damage: A phase shift characterization

Yang He, Yongda Yan, Yanquan Geng

Summary: This study investigates the use of phase shift as a sensitive measure for nondestructive surface morphology measurement using an atomic force microscope. The research found that phase shift can signify the transition from nondestructive to destructive measurement, providing valuable insights for surface characterization.

ULTRAMICROSCOPY (2023)

Article Microscopy

Investigating the effects of solution viscosity on the stability and success rate of SECCM imaging

Qiangqiang Zheng, Jian Zhuang, Tianying Wang, Xiaobo Liao, Lei Cheng, Shengbo Gu

Summary: By using finite element analysis, the influence of solution viscosity on the shape variation of the droplet at the tip during the movement of the pipette in scanning electrochemical cell microscopy (SECCM) was studied. It was proved that increasing solution viscosity is helpful in reducing the shape variation of the droplet at the tip. Experimental results showed that increasing solution viscosity improves scanning success rates and quality, and effectively lowers the mean square error (MSE) of the scanning results. The results also demonstrated that SECCM can achieve higher imaging speeds when the solution's viscosity increases.

ULTRAMICROSCOPY (2023)

Article Microscopy

Effect of Nano-selenium on exosomes secretion associated with sperm maturation within the epididymis

Chuang Zhou, Hua Zhang, Yi Wu, Nisar Ahmed

Summary: The study shows that selenium nanoparticles have a significant impact on sperm concentration and exosome secretion in the epididymis, potentially playing a crucial role in sperm motility and maturation.

MICRON (2023)

Article Microscopy

Edge-induced excitations in Bi2Te3 from spatially-resolved electron energy-gain spectroscopy

Helena La, Abel Brokkelkamp, Stijn van der Lippe, Jaco ter Hoeve, Juan Rojo, Sonia Conesa-Boj

Summary: Among the potential applications of topological insulator materials, the development of tunable plasmonics at THz and mid-infrared frequencies for quantum computing, terahertz detectors, and spintronic devices is particularly attractive. However, understanding the relationship between nanoscale crystal structure and the properties of plasmonic resonances remains elusive. In this study, energy-gain EELS analysis was used to characterize collective excitations in the topological insulator material Bi2Te3 and correlate them with the underlying crystalline structure. The findings demonstrate the potential of energy-gain EELS analysis in accurately mapping collective excitations in quantum materials, which is crucial for the development of new tunable plasmonic devices.

ULTRAMICROSCOPY (2023)