Cell & Tissue Engineering

76.5K Papers

Follow See full results

Article Cell & Tissue Engineering

Co-overexpression of OPN, IGF-1 and CNTF augment the therapeutic effect of DPSC on spinal cord injury

Ning Tao, Xiwen Dong, Chao Liu, Lin Lv, Fanqi Hu, Huan Zhang, Xue Li, Panpan Geng, Han Duan, Chu-Tse Wu, Hua Wang

Summary: This study investigated the therapeutic effect of gene-modified dental pulp stem cells (DPSCs) on spinal cord injury (SCI). The results demonstrated that DPSC-OIC enhanced cell proliferation and axon growth, protected cells from damage, and promoted functional recovery in injured spinal cord.

REGENERATIVE THERAPY (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Lipoaspirate stored at a constant low temperature by electric control suppresses intracellular metabolism and maintains high cell viability

Ryoko Inaki, Yoshihiko Sato, Daisuke Nakamura, Yoshiyuki Aikawa, Tsuyoshi Takato, Kazuto Hoshi, Atsuhiko Hikita

Summary: This study investigated the optimal temperature for the transportation of lipoaspirates from which adipose-derived stem cells (ASCs) were isolated, and found that transporting lipoaspirates at 4 degrees Celsius resulted in the best outcomes. This research provides a direction for building a transport system that connects laboratories and hospitals to achieve beneficial therapy for patients.

REGENERATIVE THERAPY (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Generation of two iPSC lines (MHHi001-A-12 and MHHi001-A-13) carrying biallelic truncating mutations at the 3′-end of SRCAP using CRISPR/Cas9

J. Rhode, L. Hagenau, J. Beimdiek, R. Ullmann, F. Hossain, A. Tzvetkova, L. R. Jensen, A. W. Kuss

Summary: This study describes a disease model for Non-Floating Harbour Syndrome (FLHS) neurodevelopmental disorder (NDD), in which iPSC lines with truncating mutations in the SRCAP gene were generated using CRISPR/Cas9 technology.

STEM CELL RESEARCH (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Generation of an NSD2-deficient human embryonic stem cell line using CRISPR/Cas9 technology

Kui Zhao, Hanyue Zhang, Jiaqi Cui, Yanqi Zhang, Tiancheng Zhou, Bing Long

Summary: In this study, a human embryonic stem cell line with knockout of NSD2 was constructed using CRISPR/Cas9-mediated gene targeting. This cell line serves as a good model for studying the roles of NSD2 in embryonic development, particularly in hematopoiesis.

STEM CELL RESEARCH (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Size control of induced pluripotent stem cells colonies in two-dimensional culture for differentiation into functional monocyte-like cells

Noriko Shimasaki, Eiko Shimizu, Yuta Nakamura, Hiroki Iguchi, Anna Ueda, Masafumi Umekage, Satoshi Haneda, Osam Mazda

Summary: A dot plate method was developed to control the size of induced pluripotent stem cell (iPSC) colonies, resulting in a reduction in the generation-variation of functional monocyte-like cells. This standardized method could facilitate the development of an automated closed system on a large scale for clinical applications.

CYTOTHERAPY (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Generation and characterization of induced pluripotent stem cell lines derived from skin fibroblasts of patients with adrenoleukodystrophy

Qiu-Hong Wang, Li-Ying Liu, Yang-Yang Wang, Wen He, Jia Wang, Jing Wang, Li-Ping Zou

Summary: This study established two strains of iPSCs as an alternative cell source for studying the pathogenesis and therapeutic strategies of X-linked adrenoleukodystrophy (ALD).

STEM CELL RESEARCH (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions

Mary Jasmine D. Lara, Sissy E. Wamaitha, Auriana Arabpour, Jon D. Hennebold, Amander T. Clark, Enrique Sosa

Summary: In this study, we generated and characterized a rhesus macaque induced pluripotent stem cell (iPSC) line, riPSC05, through induced reprogramming of fibroblasts using non-integrating Sendai virus technology. The authenticity of riPSC05 was confirmed through various evaluations, including the expression of pluripotent and self-renewal markers, directed differentiation towards three germ layers, karyotyping, and STR analysis.

STEM CELL RESEARCH (2023)

添加到收藏夹

Review Cell & Tissue Engineering

Research status of facial nerve repair

Haoyuan Huang, Qiang Lin, Xi Rui, Yiman Huang, Xuanhao Wu, Wenhao Yang, Zhu Yu, Wenpeng He

Summary: The facial nerve is crucial for controlling facial muscle movement and its damage can lead to severe functional impairment and facial paralysis. Significant progress has been made in the field of facial nerve repair in recent years, including direct suture, autologous nerve grafts, and tissue engineering methods.

REGENERATIVE THERAPY (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Successful reconstruction of the rat ureter by a syngeneic collagen tube with a cardiomyocyte sheet

Shutaro Yamamoto, Kenji Matsui, Yoshitaka Kinoshita, Hiroshi Sasaki, Hidekazu Sekine, Yatsumu Saito, Yasuhide Nakayama, Haruki Kume, Takahiro Kimura, Takashi Yokoo, Eiji Kobayashi

Summary: This study aimed to reconstruct the ureter using subcutaneously prepared autologous collagen tubes in a rat model. It was found that urinary flow supported the epithelial spread of the urothelium into the reconstructed ureter, and neonatal rat cardiomyocyte sheets supported the patency of the regenerated ureter with a layered urothelium.

REGENERATIVE THERAPY (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Establishment of MUi030-A: A human induced pluripotent stem cell line carrying homozygous L444P mutation in the GBA1 gene to study type-3 Gaucher disease

Kitsada Kangboonruang, Tanapat Pornsukjantra, Pirut Tong-Ngam, Tanida Chokpanuwat, Thipwimol Tim-Aroon, Duangrurdee Wattanasirichaigoon, Usanarat Anurathapan, Suradej Hongeng, Nithi Asavapanumas, Kanit Bhukhai, Alisa Tubsuwan

Summary: In this study, researchers generated a human induced pluripotent stem cell (hiPSC) line from a Gaucher disease patient using a non-integration method. These hiPSCs showed normal characteristics and the ability to differentiate into different cell types. This model holds promise for studying the underlying mechanisms of Gaucher disease.

STEM CELL RESEARCH (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Autologous concentrated growth factor mediated accelerated bone healing in root-end microsurgery: A multicenter randomized clinical trial*

Yoshio Yahata, Keisuke Handa, Naoto Ohkura, Motoki Okamoto, Jun Ohshima, Shusaku Itoh, Nobuyuki Kawashima, Toshinori Tanaka, Nobuya Sato, Yuichiro Noiri, Mikako Hayashi, Takashi Okiji, Masahiro Saito

Summary: This randomized multicenter trial evaluated the effects of concentrated growth factor (CGF) on bone healing in combination with root-end microsurgery. The results showed that CGF in conjunction with root-end microsurgery accelerated lesion reduction, indicating it as a promising treatment option.

REGENERATIVE THERAPY (2023)

添加到收藏夹

Review Cell & Tissue Engineering

Adipose stem cells in tissue regeneration and repair: From bench to bedside

Lei Dong, Xiaoyu Li, Wenyuan Leng, Zhenke Guo, Tianyu Cai, Xing Ji, Chunru Xu, Zhenpeng Zhu, Jian Lin

Summary: ADSCs are a type of mesenchymal stem cells found in adipose tissue, with the potential to differentiate into various tissues. They offer advantages such as abundant quantity, easy accessibility, stable function, and low immune rejection. Two main methods for tissue repair and regeneration using ADSCs include implanting ADSCs-scaffold composites and utilizing ADSC-exos or ADSC-CM to release bioactive substances. The tissue regeneration potential and differentiation of ADSCs are regulated by various factors, and further research is needed to fully understand the mechanisms.

REGENERATIVE THERAPY (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Early revascularization activates quiescent dental pulp stem cells following tooth replantation in mice

Hiroto Sano, Kuniko Nakakura-Ohshima, Angela Quispe-Salcedo, Yasuo Okada, Takuichi Sato, Hayato Ohshima

Summary: Perforation of the pulp chamber floor promotes pulpal healing and enhances the survival and differentiation of dental pulp stem cells. This study provides insights into the crosstalk mechanism between immune cells and DPSCs, and the differentiation process of DPSCs into odontoblast-like cells.

REGENERATIVE THERAPY (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Generation and characterization of iPS cell line (SYSUSHi002-A) from PBMCs of an AML patient with TP53 mutation

Lihua Yuan, Yaqun Wang, Rongli Xie, Dongjun Lin, Xiaobo Wang

Summary: In this study, we successfully generated an iPSCs line with pluripotency from AML cells of a patient with TP53 gene mutation. The iPSCs line displayed typical embryonic stem cell-like characteristics in morphology, gene expression, and functionality.

STEM CELL RESEARCH (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Generation of a human induced pluripotent stem cell line (UMGACBi001-A) from urine cells of a chronic kidney disease patient with hypertension, diabetic nephropathy and acute sepsis

Tim Lange, Sophia-Marie Bach, Thor-Magnus Koppe, Anja Klann, Anja Weise, Robin Alexander Pilz, Norina Loth, Sabrina von Rheinbaben, Sylvia Stracke, Nicole Endlich

Summary: In this study, a human induced pluripotent stem cell line was generated from urine cells of a chronic kidney disease patient using non-integrating administration of RNAs. The iPSCs exhibited typical characteristics and pluripotency marker expression, making them a valuable source for disease modeling and personalized therapeutic approaches.

STEM CELL RESEARCH (2023)

添加到收藏夹

Article Cell & Tissue Engineering

circ_SPEF2 Regulates the Balance of Treg Cells by Regulating miR-16-5p/BACH2 in Lymphoma and Participates in the Immune Response

Jie Zhou, Min Xu, Zhaozhao Chen, Linlin Huang, Zhuolin Wu, Zhongpei Huang, Lin Liu

Summary: circSPEF2 can influence DLBCL progression by managing cellular proliferation and apoptosis and the proportion of immune cells Treg and CD4 through the miR-16-5p/BACH2 axis.

TISSUE ENGINEERING AND REGENERATIVE MEDICINE (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Development of an In Vitro Model for Inflammation Mediated Renal Toxicity Using 3D Renal Tubules and Co-Cultured Human Immune Cells

Mi-lang Kyun, Tamina Park, Hyewon Jung, Inhye Kim, Ji-In Kwon, Seo Yule Jeong, Myeongjin Choi, Daeui Park, Yu Bin Lee, Kyoung-Sik Moon

Summary: In this study, an in vitro kidney toxicity model was developed by co-culturing 3D engineered kidney proximal tubules cells with human peripheral blood mononuclear cells, mimicking the human immune system. The results showed that induced hyperinflammation resulted in reduced primary cilia formation and upregulation of kidney injury markers. This model is suitable for detecting immune-associated kidney damage resulting from infections and biopharmaceutical applications.

TISSUE ENGINEERING AND REGENERATIVE MEDICINE (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Generation of an NCS1 gene knockout human induced pluripotent stem cell line using CRISPR/Cas9

Smilla K. Maierhof, Christian Schinke, Janine Cernoch, Lois Hew, Laura Pauline Bruske, Valeria Fernandez Vallone, Kristin Fischer, Harald Stachelscheid, Petra Huehnchen, Matthias Endres, Sebastian Diecke, Narasimha Swamy Telugu, Wolfgang Boehmerle

Summary: In this study, a NCS1 knockout human induced pluripotent stem cell (hiPSC) line was generated using CRISPR-Cas9 genome editing. The newly generated cell line provides a valuable tool for studying the role of NCS1 in the pathophysiology of various neuropsychiatric disorders and non-neurological disease.

STEM CELL RESEARCH (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Generation of iPSC lines with SLC16A2:G401R or SLC16A2 knock out

Katarzyna Anna Ludwik, Robert Opitz, Sabine Jyrch, Matthias Megges, January Weiner, Dieter Beule, Peter Kuehnen, Harald Stachelscheid

Summary: Using CRISPR/Cas9 technology, the missense variant and novel knock-out deletion variant of the SLC16A2 gene associated with Allan-Herndon-Dudley syndrome were introduced into healthy donor cells, leading to the successful generation of cerebral organoids to model the effects of MCT8 deficiency on human neurodevelopment.

STEM CELL RESEARCH (2023)

添加到收藏夹

Article Cell & Tissue Engineering

Generation of induced pluripotent stem cell line (TMOi001-A-11) carrying a homozygous deletion in the synemin gene using CRISPR/Cas9

Caterina Gargano, Jean-Sebastien Vartanian-Grimaldi, Yeranuhi Hovhannisyan, Pierre Joanne, Onnik Agbulut, Zhenlin Li, Ara Parlakian, Jean-Francois Decaux

Summary: A human induced pluripotent stem cell line with a homozygous deletion in the SYNM gene has been generated using CRISPR/Cas9 genome editing. This cell line exhibits typical characteristics of pluripotent cells and normal differentiation capacity, making it suitable for studying the role of synemin in cardiomyopathy.

STEM CELL RESEARCH (2023)

添加到收藏夹

«
1
2
3
4
5
»

See full results

Related Categories

Biochemical Research Methods (374.2K) Biochemistry & Molecular Biology (1.8M) Biology (481.8K) Biophysics (441.3K) Biotechnology & Applied Microbiology (693.1K) Cell Biology (984.6K) Development Studies (51.9K) Developmental Biology (136.9K) Entomology (167.7K) Evolutionary Biology (162.2K) Genetics & Heredity (622.4K) Marine & Freshwater Biology (270.7K) Microbiology (574.9K) Microscopy (30.5K) Mycology (57.2K) Neuroimaging (69.7K) Neurosciences (1.3M) Ornithology (39.7K) Parasitology (124.7K) Physiology (328.7K) Plant Sciences (650.5K) Reproductive Biology (204.5K) Respiratory System (555.1K) Sport Sciences (326.8K) Virology (182.8K) Zoology (363.4K)

© Peeref 2019-2024. All rights reserved.