Article
Cell & Tissue Engineering
Daiki Kitano, Yasuyuki Morimatsu, Nobuyuki Murai, Takeo Osaki, Shunsuke Sakakibara
Summary: This study retrospectively investigated the relationship between the superficial branch of the radial nerve (SBRN) preservation method and post-operative sensation at the flap donor-site. The results showed that the zero preservation method had a higher risk of subjective symptoms and objective hypoesthesia development compared to the complete preservation method. Main trunk preservation prevented the development of subjective symptoms. Complete preservation is optimal for RF flap harvest, but main trunk preservation can be considered in cases of perforator crossing.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Yunfeng Zhang
Summary: This study investigated the clinical effects of sodium hyaluronate combined with platelet-rich plasma (PRP) injection in arthroscopic repair of rotator cuff injury. The results showed that this combined injection can effectively reduce pain symptoms, improve shoulder joint function and range of motion, and enhance patients' quality of life.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Tsutomu Nakazawa, Ryosuke Maeoka, Takayuki Morimoto, Ryosuke Matsuda, Mitsutoshi Nakamura, Fumihiko Nishimura, Shuichi Yamada, Ichiro Nakagawa, Young -Soo Park, Toshihiro Ito, Hiroyuki Nakase, Takahiro Tsujimura
Summary: Natural killer cells (NKCs) can attack cancer cells through direct recognition of ligands without prior sensitization. Cord blood-derived NKCs (CBNKCs) have potential for allogeneic NKC-based cancer immunotherapy. Efficient NKC expansion and decreased T cell inclusion are crucial for successful immunotherapy without graft-versus-host reactions. This study evaluated the performance of an NKC expansion system using cord blood and characterized the expanded populations.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Takeshi Kikuchi, Masuhiro Nishimura, Chikage Shirakawa, Yasutaka Fujita, Takeshige Otoi
Summary: This study investigated the storage conditions that lead to cell aggregation and the conditions that inhibit cell aggregation when hADSCs are stored in LR-3T-5D. The results showed that storage temperature and time did not affect viability but significantly increased cell aggregation rate when stored at 25°C for 24 hours. Increasing solution volume and cell density, as well as nitrogen gas replacement, suppressed cell aggregation.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Luis Oliveros Anerillas, Mikael Wiberg, Paul J. Kingham, Peyman Kelk
Summary: The use of human platelet lysate (PLT) as an alternative to foetal bovine serum (FBS) can enhance the proliferation of mesenchymal stem cells (MSCs), but it impairs the differentiation capacity of the cells in 3D culture systems.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Liang Tan, Zhuofeng Ye, Weida Zhuang, Beini Mao, Hetong Li, Xiuwang Li, Jiachang Wu, Hongxun Sang
Summary: This study investigated the effects of polydopamine-coated poly lactic-co-glycolic acid-magnesium oxide scaffolds on bone marrow mesenchymal stem cells osteogenic differentiation. The results showed that the PLGA/MgO (10%) scaffolds had good hydrophilicity and biocompatibility, and promoted the osteogenic differentiation of BMSCs.
REGENERATIVE THERAPY
(2023)
Correction
Cell & Tissue Engineering
Chiara Lattuada, Serena Santangelo, Silvia Peverelli, Philip McGoldrick, Ekaterina Rogaeva, Lorne Zinman, Georg Haase, Vincent Geli, Vincenzo Silani, Janice Robertson, Antonia Ratti, Patrizia Bossolasco
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Shekhar Singh, Lidiia Plotnikova, Kalle Karvonen, Sanna Ryytty, Jelena Hypponen, Reetta Kalviainen, Riikka H. Hamalainen
Summary: In this study, iPSCs were generated from the skin fibroblasts of an EPM1 patient and demonstrated the potential to serve as an in-vitro model for studying the mechanisms of EPM1 at the cellular and molecular level.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Anne Kayser, Sven Dittmann, Andreas van Impel, Tomo Saric, Eric Schulze-Bahr
Summary: Researchers generated a mutant hiPSC line from patient-derived cells, which could serve as an in vitro model to study sinus node dysfunction and develop pharmacological rescue strategies.
STEM CELL RESEARCH
(2023)
Review
Cell & Tissue Engineering
Ying-tong Wang, Xiao-ting Meng
Summary: Tissue engineering is a promising solution for regenerative medicine, and its success depends on rapid vascularization to supply oxygen and nutrients to cells. Developing technologies to facilitate vascularization of engineered tissues is a current focus. This review indicates that a regulatory system influencing angiogenesis-associated cells is ideal for constructing vascularized engineered tissues, and electrical stimulation (ES) can enhance co-cultured angiogenesis-associated cells and regulate endothelial cells (ECs) to achieve tissue vascularization. The use of ES with mesenchymal stem cells (MSCs) and ECs shows potential advantages in tissue vascularization, and the different biological pathways of ES-regulated ECs are highlighted. Future directions include the use of ES to reconstruct tissue blood vessels, along with potential advantages and disadvantages.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Raheleh Farahzadi, Zohreh Sanaat, Ali Akbar Movassaghpour-Akbari, Ezzatollah Fathi, Soheila Montazersaheb
Summary: Breast cancer stem cells (BCSCs) are a small subset of breast cancer cells that contribute to metastasis, recurrence, and drug resistance. Targeting BCSCs is a promising strategy for the treatment and prevention of breast cancer metastasis. In this study, the impact of L-carnitine (LC) on the proliferation rate and induction of apoptosis in CD44+ CSCs was investigated.
REGENERATIVE THERAPY
(2023)
Review
Cell & Tissue Engineering
Ryoma Endo, Shinya Sugimoto, Koji Shirosaki, Hirochika Kato, Motoshi Wada, Takanori Kanai, Toshiro Sato
Summary: Short bowel syndrome (SBS) is a rare condition characterized by malabsorption after extensive resection of the small intestine. The current treatment options are mainly supportive, but novel regenerative therapeutic approaches using intestinal organoids show promise for the future. Despite some challenges and issues, progress is being made in overcoming the obstacles in establishing the complex function and structure of the small intestine. This article discusses the current treatments for SBS, the fundamentals of the intestine and organoids, the current status of these new technologies, and future perspectives.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Yue Wang, Haiping Yu, Hongming Tang, Rong Zhu, Yiqi Shi, Changqin Xu, Yan Li, Hua Wang, Yuanyuan Chen, Peichen Shen, Jinfang Xu, Congrong Wang, Zhongmin Liu
Summary: This study investigated the safety evaluation of intravenous infusion of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) and found that the process was safe and vital signs were stable, but there was a slight decrease in blood pressure during infusion.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Friederike C. Messner, Wolfgang Metzger, Julia E. Marschall, Caroline Bickelmann, Michael D. Menger, Matthias W. Laschke
Summary: MVF isolates generated by short-term enzymatic digestion are beneficial for tissue defect vascularization, isolates from obese mice lack connective tissue and exhibit improved in vivo vascularization.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Cell & Tissue Engineering
Yu Ri Hong, Tae-Ho Kim, Kyueui Lee, Jeong Ok Lim, Chang-Wug Oh
Summary: A novel bioactive bone substitute was created using advanced technologies and evaluated for its safety and efficacy in a preclinical study. The results showed that the bone substitute had ideal shape and mechanical properties, as well as good biocompatibility and effective new bone formation ability.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Review
Cell & Tissue Engineering
Alejandro Reina-Mahecha, Martine J. Beers, Hugo C. van der Veen, Inge S. Zuhorn, Theo G. van Kooten, Prashant K. Sharma
Summary: This review summarizes the current understanding of bioreactors and their potential use in cartilage tissue engineering, with a focus on induced pluripotent stem cells (iPSCs). Bioreactors play a crucial role in simulating the joint cavity niche, promoting cell differentiation, and maintaining homeostasis in cartilage tissue engineering. Incorporating iPSCs and bioreactors shows promise for advancing the field and translating these approaches into clinical applications.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2023)
Article
Cell & Tissue Engineering
Qiannan Zhao, Shuichi Ogino, Sunghee Lee, Yuki Kato, Yuanjiaozi Li, Michiharu Sakamoto, Hiroki Yamanaka, Takashi Nakano, Eiichi Sawaragi, Naoki Morimoto
Summary: Poly-L-lactic acid (PLLA) mesh implants combined with collagen sponge (CS) were replaced with autologous adipose tissue for regeneration. The study compared different combinations of external frames (P (LA/CL), PLLA, LMWPLLA) and internal fillings (PGA nanosheets, CS) and found that PLLA served as a promising external frame material while PGA nanosheets were an effective alternative internal filling material for adipose tissue regeneration.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Jincheng Zhang, Hengan Ge, Jun Li, Liyang Chen, Jiaqi Wang, Biao Cheng, Zhitao Rao
Summary: Controlled release and administration of multifunctional therapeutic agents is an effective strategy for treating nerve injury. In this study, a dual-delivery three-dimensional nanofibrous matrix loaded with melatonin and nerve growth factor was developed for peripheral nerve regeneration. The matrix promoted adipose-derived stem cell differentiation and improved sciatic nerve regeneration in rats, demonstrating its potential for peripheral nerve regeneration.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Tamaki Wada, Taichi Takenawa, Natsuki Komori, Masuhiro Nishimura, Yasutaka Fujita, Osamu Sawamoto
Summary: Researchers found that after 72 hours of cold preservation in a solution with added Vitamin C, the quality, viability, and differentiation potential of adipose-derived mesenchymal stem cells (hADSCs) remained unchanged.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Rodolfo Tonin, Federica Feo, Silvia Falliano, Lorenzo Ferri, Laura Giunti, Martino Calamai, Elena Procopio, Francesco Mari, Valerio Conti, Ilaria Fanelli, Franco Bambi, Renzo Guerrini, Amelia Morrone
Summary: In this study, a hiPSC line was generated from a 15-year-old male patient with CDG and characterized, showing normal characteristics and potential, with analysis on the variants in the ALG8 gene.
STEM CELL RESEARCH
(2023)
