Article
Agricultural Engineering
Rui Huang, Mingyue Geng, Shanshan Gao, Xing Yin, Jiayu Tian
Summary: This study aimed to alleviate membrane fouling and improve nitrogen removal by applying high sludge concentration in MBR. The results showed that high sludge concentration enhanced total nitrogen removal and reduced pressure development rate. Microbial community analysis revealed the enrichment of functional bacteria associated with nitrogen removal and the inhibition of Thiothrix overgrowth. Moreover, key genes involved in nitrogen metabolism and amino acid degradation were up-regulated under high sludge concentration. Overall, high sludge concentration improved microbial assembly and functional gene abundance, which enhanced nitrogen removal and alleviated membrane fouling.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Mingxiao Zeng, Zhanfeng Li, Yongli Liu, Yuanyuan Wang, Xunfeng Xia, Shengwang Gao, Guangqing Song
Summary: This study introduced filler-enhanced soil infiltration systems and achieved efficient denitrification and reduction of greenhouse gas emissions in rural domestic sewage treatment.
BIORESOURCE TECHNOLOGY
(2024)
Review
Agricultural Engineering
Chenming Dai, Feifei Wang
Summary: This review focuses on the application of microalgae-bacteria consortia (MBC) in wastewater treatment and bio-product production, emphasizing the interaction mechanisms between microalgae and bacteria. It also summarizes the use of MBC in wastewater treatment and biorefinery.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Yingchao Zhang, Liqian Liu, Guowei Huang, Changhao Yang, Wenxin Tian, Zhenyu Ge, Baohai Zhang, Sufeng Wang, Hongqiong Zhang
Summary: This study investigated the effects of biochar and Fe2O3 on the co-composting of pig manure and wine grape pomace. The results showed that Fe2O3 enhanced microbial network symbiosis, increased the yield of humic acid and germination index, while biochar increased microbial interactions without significant effect on humic acid yield. Bacillus and Actinomadura were identified as pivotal factors affecting humic acid content.
BIORESOURCE TECHNOLOGY
(2024)
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Agricultural Engineering
Meenaa Chandrasekar, Jeana L. Collins, Sanaz Habibi, Rebecca G. Ong
Summary: The tissue-specific biochemical heterogeneities of lignocellulosic biomass and their effect on biomass deconstruction can be best understood through the combination of confocal laser scanning microscopy (CLSM) and immunohistochemistry. A custom polydimethylsiloxane (PDMS) microfluidic imaging reactor was developed to overcome the challenges of studying fragile plant materials. The results showed that less lignified parenchyma cell walls are more susceptible to pretreatment, while highly lignified protoxylem cell walls are the most resistant during enzymatic hydrolysis.
BIORESOURCE TECHNOLOGY
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Sijie Yin, Zhiqiang Yu, Ningning Song, Zhanjun Guo, Wei Li, Jinhang Ma, Xiaoyan Wang, Junyao Liu, Minmin Liang
Summary: Researchers have developed a microneedle-based glucose monitoring skin patch that can continuously and accurately monitor glucose levels in real time, offering high sensitivity and a long lifespan. The results can be displayed on a smartphone.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minsu Jang, Jonghoon Shin, You Hwan Kim, Tae-Young Jeong, Soojin Jo, Sung-Jo Kim, Vasanthan Devaraj, Joonhee Kang, Eun-Jung Choi, Ji Eun Lee, Jin-Woo Oh
Summary: Metabolome analysis using 3D superstructures has been applied for glaucoma diagnosis, providing comprehensive information and accurate classification of patients. The advantages of 3D superstructures, including high hotspot density, excellent signal repeatability, and thermal stability, enable versatile metabolic analysis and disease diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lanlan Xu, Mo Ma, Jingkang Li, Dianfeng Dai, Dejiang Gao, Pinyi Ma, Qiong Wu, Daqian Song
Summary: This study developed an ultra-sensitive probe, LAN-apn, to investigate the expression level of APN in thyroid cancer and evaluate its potential as a biomarker. The results suggest that APN may become a new biomarker for thyroid cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chunlian Qin, Qunchen Yuan, Mengxue Liu, Liujing Zhuang, Lizhou Xu, Ping Wang
Summary: This study proposes a biohybrid tongue based on hypothalamic neuronal network coupled with microelectrode array for real-time glucose perception. Hypothalamic neuronal cultures were cultivated on a two-dimensional brain-on-chip device, enabling the formation of neuronal networks and electrophysiological signal detection. The study investigates the endogenous expression of sweet taste receptors in hypothalamic neuronal cells and explores the spike signal response to sucrose and glucose stimulation. The biohybrid tongue provides insights into glucose sensing mechanisms and hypothalamic neuronal function.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Edward J. Jacobs, Kenneth N. Aycock, Pedro P. Santos, Joanne L. Tuohy, Rafael V. Davalos
Summary: The efficiency of electroporation treatments depends on the application of a critical electric field over the targeted tissue volume. A deep neural network trained on finite element model simulations was developed to predict tissue properties from a measured voltage ramp, showing minimal test error and great utility in clinical applications. The platform can quickly ascertain patient-specific tissue properties required for treatment planning.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Qi Xu, Jinze Li, Zhiqi Zhang, Qi Yang, Wei Zhang, Jia Yao, Yaxin Zhang, Yueye Zhang, Zhen Guo, Chao Li, Shuli Li, Changsong Zhang, Chuanxin Wang, Lutao Du, Chuanyu Li, Lianqun Zhou
Summary: Real-time digital polymerase chain reaction (qdPCR) integrates absolute quantification with process information, improving precision in molecular diagnostics. We propose a novel approach to determine optimal reaction conditions for qdPCR using real-time fluorescence monitoring in microwells. By leveraging an ultrathin laminated chip (UTL chip), we achieve faster amplification and higher accuracy, showing promise for molecular diagnostics.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
M. Mahabubur Rahman, Nabil H. Bhuiyan, Minjun Park, M. Jalal Uddin, Gyeong J. Jin, Joon S. Shim
Summary: In this work, a novel and low-cost fabrication technology for interdigitated electrode array (IDA) electrodes on the polymer substrate was developed. The fabricated IDA electrode showed high sensitivity and detection limit in electrochemical detection of biomarkers.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Jun Wang, Jiawen Han, Juan Wang, Xujuan Lv, Daoqing Fan, Shaojun Dong
Summary: This study reports a ratiometric fluorescent platform based on nanozymes for the highly sensitive detection of cysteine and bleomycin. The platform exhibits acceptable performance and high selectivity, and shows good performance in human serum samples. Additionally, DNA contrary logic pairs and multilevel concatenated circuits were fabricated, enriching the building blocks of biocomputing.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Yinru Zhu, Benqing Wu, Xinwei Gao, Yong Guo, Xiao Peng, Luwei Wang, Xuewu Tian, Jian Wang, Wei Yan, Hui Zhang, Junle Qu
Summary: Preeclampsia is a common pregnancy complication and the leading cause of maternal and perinatal mortality. However, early diagnostic methods for preeclampsia are still rare. This study explores the possibility of using fluorescence lifetime imaging and the Nile Blue probe for the early diagnosis and severity prediction of preeclampsia.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Bettina Glahn-Martinez, Beatriz Jurado-Sanchez, Elena Benito-Pena, Alberto Escarpa, Maria C. Moreno-Bondi
Summary: This study developed a novel bioassay for monitoring FK506 using magnetic Janus micromotors and a recombinant chimera receptor. The bioassay utilized a fluorescence competitive method between the drug and the micromotors to achieve a one-step assay without washing steps. The method exhibited a high limit of detection and dynamic range, and was able to operate in raw samples.
BIOSENSORS & BIOELECTRONICS
(2024)
