Biotechnology & Applied Microbiology

Article Agricultural Engineering

Enhanced adsorption of aromatic VOCs on hydrophobic porous biochar produced via microwave rapid pyrolysis

Junhao Lin, Zibo Xu, Qiaozhi Zhang, Yang Cao, Ondr ej Mas, Hanwu Lei, Daniel C. W. Tsang

Summary: This study presents a method of programmed microwave pyrolysis combined with low-dose ferric chloride to produce hydrophobic porous biochar suitable for efficient adsorption of benzene derivatives. The results show that programmed microwave pyrolysis can significantly increase the specific surface area of biochar and enhance its adsorption capacity for benzene and toluene.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Machine learning-based prediction of methane production from lignocellulosic wastes

Chao Song, Fanfan Cai, Shuang Yang, Ligong Wang, Guangqing Liu, Chang Chen

Summary: This paper developed a machine learning model to predict the biochemical methane potential during anaerobic digestion. Model analysis identified lignin content, organic loading, and nitrogen content as key attributes for methane production prediction. For feedstocks with high cellulose content, early methane production is lower but can be improved by prolonging digestion time. Moreover, lignin content exceeding a certain value significantly inhibits methane production.

BIORESOURCE TECHNOLOGY (2024)

Article Biotechnology & Applied Microbiology

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery

Yujun Zhai, Weina Tian, Kewei Chen, Linshu Lan, Jianquan Kan, Hui Shi

Summary: This research investigates the role of flagella-related genes in the adhesion of E. coli O157:H7 and the effects of certain cations on bacterial adhesion and motility. The results demonstrate that flagellar-mediated motility is critical in the early stage of adhesion, and the presence of Fe2+, Ca2+, and Mn2+ affects the adhesion and motility of wildtype strains but not flagellar function-impaired strains. These findings provide new insights into the mechanisms of bacterial adhesion and have implications for the development of anti-adhesion agents.

FOOD MICROBIOLOGY (2024)

Article Biotechnology & Applied Microbiology

CpxAR two-component system contributes to virulence properties of Cronobacter sakazakii

Tong Jin, Xiangjun Zhan, Liuxin Pang, Bo Peng, Xinpeng Zhang, Wenxiu Zhu, Baowei Yang, Xiaodong Xia

Summary: The study found that CpxAR TCS is an important regulatory mechanism for the virulence of C. sakazakii. Mutants showed weakened adhesion, invasion, and survival ability, and exhibited attenuated virulence in animal models. In addition, CpxAR TCS is involved in regulating the expression of genes associated with bacterial virulence and cellular invasion.

FOOD MICROBIOLOGY (2024)

Article Biochemical Research Methods

Nano-emulsion encapsulation for the efficient delivery of bacteriophage therapeutics

Loushambam Samananda Singh

Summary: Antibiotic resistance has become a major concern for global public health, and phage therapy is seen as an alternative for treating multidrug resistant bacterial infections. Nano-emulsion encapsulation is described as the best approach to maintain phage efficacy.

BIOLOGICALS (2024)

Article Biochemical Research Methods

An archaeal nitrile hydratase from the halophilic archaeon A07HB70 exhibits high tolerance to 3-cyanopyridine and nicotinamide

Junling Guo, Zhongyi Cheng, Zhemin Zhou

Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.

PROTEIN EXPRESSION AND PURIFICATION (2024)

Article Agricultural Engineering

The influencing mechanism of AD-MEC domesticated sludge to alleviates propionate accumulation and enhances methanogenesis

Meixin Guo, Meng Guo, Yong Wang, Mingxiao Li, Xuejiao Qi, Sijia Wei, Xuan Jia

Summary: This study investigates the addition of sludge to AD-MEC to improve system performance and explore microbial interactions after electric field domestication. The results show that the addition of sludge increases methane production and enhances the sludge's continuous methanogenic capacity. Further analysis reveals that the sludge in the AD-MEC system has enhanced extracellular electron transfer capacity and forms a microbial community with synergistic acid degradation and methanogenesis capabilities.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Effect of turbulent diffusion in modeling anaerobic digestion

Jeremy Z. Yan, Prashant Kumar, Wolfgang Rauch

Summary: This study investigates the impact of turbulent diffusion on mixing of biochemical reaction models and develops a new codebase called CHAD to incorporate and validate turbulent diffusion. The findings show that the implemented models accurately capture turbulent diffusion and reveal that thermal turbulent diffusion significantly influences mixing.

BIORESOURCE TECHNOLOGY (2024)

Review Agricultural Engineering

Melatonin, a phytohormone for enhancing the accumulation of high-value metabolites and stress tolerance in microalgae: Applications, mechanisms, and challenges

Yongteng Zhao, Qingwei Wang, Dan Gu, Feiyan Huang, Jiani Liu, Lei Yu, Xuya Yu

Summary: This review highlights the role of melatonin in promoting the accumulation of high-value metabolites and enhancing stress resistance in microalgae. It discusses the underlying mechanisms of melatonin and offers new perspectives on utilizing it to improve microalgal productivity and stress resistance in challenging environments.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

NADH-dependent formate dehydrogenase mutants for efficient carbon dioxide fixation

Yaju Xue, Xiuling Ji, Zhuang Li, Fuqiang Ma, Jingjie Jiang, Yuhong Huang

Summary: This study developed two recombinant mutants with enhanced CO2 reduction activity, leading to improved efficiency in converting CO2 to methanol. Molecular dynamics analysis revealed that increasing the width of the substrate pocket and enhancing the rigidity of C-terminal residues were key factors in improving the enzyme's reduction activity.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Performance and mechanism of pilot-scale carbon fibers enhanced ecological floating beds for urban tail water treatment in optimized ecological floating beds water surface coverage

Zhengyu Yan, Dahong Chen, Ye Qiu, Dongyi Li, Chen Yan, Jiannan Li, Da Li, Guohong Liu, Yujie Feng

Summary: A pilot-scale carbon fibers enhanced ecological floating beds (CF-EFBs) were constructed and found to have better removal efficiency for nitrogen, phosphorus, and chemical oxygen demand compared to non-enhanced EFBs. The concentrations of COD, TIN, and TP in the effluent were 18.11 +/- 4.52 mgL-1, 1.95 +/- 0.92 mgL-1, and 0.13 +/- 0.08 mgL-1 respectively, while the average removal of TIN, TP, and COD from tailwater was 0.96 gm- 2d-1, 0.07 gm- 2d-1, and 2.37 gm- 2d-1 respectively.

BIORESOURCE TECHNOLOGY (2024)

Article Biotechnology & Applied Microbiology

Pediococcus inopinatus with a well-developed CRISPR-Cas system dominates in long-term fermented kimchi, Mukeunji

So Yeong Mun, Wooje Lee, Soo-Young Lee, Ji Yoon Chang, Hae Choon Chang

Summary: This study reveals that lactobacilli with a strong defense system dominate the final stage of kimchi fermentation and proposes a model for the succession mechanism of kimchi lactobacilli.

FOOD MICROBIOLOGY (2024)

Article Biotechnology & Applied Microbiology

Inactivation of E. coli O157:H7, Salmonella enterica, and L. monocytogenes through semi-continuous superheated steam treatment with additional effects of enhancing initial germination rate and salinity tolerance

Soo-Hwan Kim, Sang-Hyun Park, Jun-Bae Ahn, Dong-Hyun Kang

Summary: In this study, a semi continuous SHS system was developed to inactivate foodborne pathogens on radish seed surfaces and enhance the seeds' salinity tolerance. The results showed that higher temperature and more treatments led to a significant reduction in microbial count on the seed surface.

FOOD MICROBIOLOGY (2024)

Article Agricultural Engineering

Responses of greenhouse gas emissions to aeration coupled with functional membrane during industrial-scale composting of dairy manure: Insights into bacterial community composition and function

Chen Fang, Ya Su, Qianting Zhuo, Xiaoli Wang, Shuangshuang Ma, Muqing Zhan, Xueqin He, Guangqun Huang

Summary: This study evaluated the effects of different aeration and semi-permeable membrane-covered strategies on bacterial communities, greenhouse gas emissions, and metabolic functions. The results showed that forced aeration coupled with semi-permeable membrane-covered was an effective strategy for reducing greenhouse gas emissions.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Achieving rapid endogenous partial denitrification by regulating competition and cooperation between glycogen accumulating organisms and phosphorus accumulating organisms from conventional activated sludge

Zhihao Peng, Qiong Zhang, Xiyao Li, Shouyou Gao, Caifang Jiang, Yongzhen Peng

Summary: In this study, steady endogenous partial denitrification (EdPD) was achieved by regulating the competition and cooperation between microorganisms, resulting in a balanced phosphorus removal efficiency and nitrite transformation rate, which contributed to advanced nitrogen removal.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Electrodialysis treatment of rhamnolipids hydrolysate and its waste water for use as water-soluble fertilizer

Chong Shen, Yizeng Li, Gang Lu, Qin Meng

Summary: This study introduced electrodialysis technology as an alternative method for purifying rhamnolipids hydrolysate, significantly reducing wastewater generation and repurposing it into water-soluble fertilizer. This fertilizer with various nutritional components showed promising effects in enhancing tomato plant growth and fruit quality. The electrodialysis treatment has the potential for reducing water volume and economic costs, making it suitable for large-scale rhamnose production.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Feast/famine ratio regulates the succession of heterotrophic nitrification-aerobic denitrification and autotrophic ammonia oxidizing bacteria in halophilic aerobic granular sludge treating saline wastewater

Yuan Sui, You-Wei Cui, Ji-Lin Huang, Meng-Jiao Xu

Summary: The feast/famine ratio was found to regulate the population succession of autotrophic ammonia oxidizing bacteria (AOB) and heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria, providing a solution for enriching HN-AD bacteria in halophilic aerobic granular sludge (HAGS) systems.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Insight into coagulation/flocculation mechanisms on microalgae harvesting by ferric chloride and polyacrylamide in different growth phases

Siqi Zhang, Jingyi Cao, Yajiao Zheng, Meifang Hou, Lili Song, Jiandie Na, Yiqiang Jiang, Yichen Huang, Tianyi Liu, Hua Wei

Summary: The effect of coagulation, flocculation, and their combination using FeCl3 and PAM on algae cells and extracellular organic matter (EOM) was investigated. FeCl3 + PAM exhibited the highest removal efficiency during stationary phase I.

BIORESOURCE TECHNOLOGY (2024)

Article Agricultural Engineering

Mechanisms of total phosphorus removal and reduction of β-lactam antibiotic resistance genes by exogenous fungal combination activated sludge

Yixiang Cai, Feng Liu, Guiyi He, Xiaoliang Kong, Yuexi Jiang, Ji Liu, Binghua Yan, Shunan Zhang, Jiachao Zhang, Zhiyong Yan

Summary: This study used Trichoderma and activated sludge to construct combined activated sludge (TAS) and employed a metagenomic approach to investigate the shift in microbial community structure and function of TAS under amoxicillin stress. The findings revealed that increased abundance of certain genes contributed to the reduction in total phosphorus removal by TAS, while increased abundance of specific bacterial phyla improved TP removal. Furthermore, antibiotic inactivation has become the primary antibiotic resistance mechanism in TAS.

BIORESOURCE TECHNOLOGY (2024)

Article Biotechnology & Applied Microbiology

Environmental implications of bacterial-derived extracellular reactive oxygen species

Shaofeng Zhou, Wenwen An, Cuifen Gan, Meiying Xu

Summary: Bacteria generate and release extracellular reactive oxygen species (eROS), which significantly contribute to the natural ROS pool and play a crucial role in metabolic interactions of bacteria with their environment. Understanding the behavior of eROS-generating bacteria and its environmental implications is of great importance in the cycling of biogeochemical elements, transformation of organic matter, and regulation of antibiotic resistance genes.

INTERNATIONAL BIODETERIORATION & BIODEGRADATION (2024)