Article
Biotechnology & Applied Microbiology
Thore Bach Thomsen, Tobias S. Radmer, Anne S. Meyer
Summary: Plastic pollution, particularly from PET, is a significant environmental challenge. This study investigates the enzyme kinetics of PET degradation and finds that the degree of crystallinity (X-C) has a substantial impact on the enzymatic reaction rate. The mobile amorphous fraction (X-MAF) is a better descriptor for the rate of enzymatic degradation. The longer lag phase observed with increased X-C is proposed to be due to increased substrate entanglement density and unproductive enzyme binding to the crystalline regions of PET.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biochemical Research Methods
Neetu Raghav, Seema Parveen, Simleen Kaur, Selwyn A. Wilson David, Hyesuk Kong, James L. Kenney, Rajesh K. Gupta
Summary: This study evaluated the use of solid medium in sterility testing. Results showed that the sterility test using Schaedler blood agar (SBA) was more sensitive and faster in detecting microorganisms compared to the current compendial method, particularly in samples containing thimerosal. The sterility test using solid medium could be completed in 7 days without any additional technology.
Article
Agricultural Engineering
Shijia Dong, Yiyang Wei, Qi Yu, Yunfei Gao, Heshu Chen, Kaiyun Zhou, Meidi Cheng, Bo Wang, Yuquan Wei, Xiaomei Hu
Summary: This study compared the effects of functional inoculant and different nitrogen sources on straw composting. The results showed that inoculation improved the heating process and increased the formation of humus. Bioinformatics analysis revealed different bacterial genera in relation to different nitrogen sources, and network analysis showed enhanced microbial synergistic effect with inoculation and nitrogen addition.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Yi-Lu Sun, Jia-Yu Wang, Huu Hao Ngo, Wei Wei, Wenshan Guo, Xue-Ning Zhang, Hao-Yi Cheng, Ji-Xian Yang, Ai-Jie Wang
Summary: This study investigates the advantages and problems of sulfur fluidizing bioreactor (S0FB) in treating nitrate-rich wastewater. It reveals that self-acidification is the main problem and can be addressed by providing sufficient alkalinity. The experimental results show that S0FB has higher nitrogen and phosphate removal rates compared to conventional sulfur packing bioreactors.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ruoyan Li, Hui Ruan, Daihui Zhang, Chongyang Zhu, Chenhuan Lai, Qiang Yong
Summary: In this study, a combined pretreatment involving autohydrolysis and p-toluenesulfonic acid (p-TsOH) was performed on poplar to coproduce xylooligosaccharides (XOSs) and monosaccharides. The enzymatic digestibilities of the combined pretreated substrates were found to be extremely poor, but the addition of Tween 80 successfully improved the enzymatic hydrolysis.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Hu Shu, Yonghao Ma, Huijie Lu, Huiming Sun, Jichen Zhao, Zhuohao Ruan, Jiayi Zhou, Yuting Liu, Fengkun Liu, Jingxuan Xu, Yazhi Zheng, Hui Guo, Qionghua Chen, Wen Huang
Summary: Pseudomonas mendocina A4 strain has the ability of simultaneous heterotrophic nitrification-aerobic denitrification and phosphorus removal. It shows adaptability to pH, carbon/nitrogen ratio, phosphorus/nitrogen ratio, temperature, and salinity. The nitrogen and phosphate removal processes follow the Gompertz model and are confirmed by the activities of key metabolic enzymes. Immobilization technology can enhance the nitrogen and phosphate removal efficiencies of strain A4. These findings suggest the potential application of strain A4 in both freshwater and marine culture wastewater treatment.
BIORESOURCE TECHNOLOGY
(2024)
Review
Biochemical Research Methods
Pei-Ru Chen, Peng-Fei Xia
Summary: Carbon dioxide plays a crucial role in mitigating global climate change and supporting life on Earth. Synthetic biology and metabolic engineering offer a promising approach to enhance CO2 fixation by combining the advantages of autotrophic and heterotrophic organisms. This article reviews the current progress in constructing synthetic CO2 fixation pathways, discusses the underlying design principles and challenges, and provides insights into the future of carbon recycling through engineered synthetic carbon fixation pathways.
CURRENT OPINION IN BIOTECHNOLOGY
(2024)
Article
Agricultural Engineering
Yi Wang, Xinru Zhang, Yuyang Wu, Guangpu Sun, Zeyi Jiang, Siyuan Hao, Shiya Ye, Hu Zhang, Fan Zhang, Xinxin Zhang
Summary: The coculture of microalgae has the potential to promote the growth of microalgae biofilms. This study found that biomass yields can decrease when the biofilm porosity decreases, but can increase when the cocultured biofilms have a uniform microstructure and small cell-clusters. Analysis of cell morphologies and surface properties revealed that cells with small size, spherical shape, and reduced surface polymers can hinder the formation of cell-clusters, thereby promoting biomass yields.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Junchi Li, Fan Zhang, Bin Ma, Dedong Kong, Yiming Hu, Guangsuo Chen, Yunjie Ruan
Summary: This study established a method for simultaneous nitrate and ammonium removal using an airlift sequencing batch reactor with 3-hydroxybutyrate-co-3-hydroxyvalerate as carbon source and biofilm carrier. The results showed high removal rates of nitrate and ammonia nitrogen with no significant nitrite accumulation.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Mari Sinisalu, Oliver Jarvik, Birgit Mets, Alar Konist
Summary: Co-gasification of biomass with oil shale can reduce reliance on fossil fuels and enhance integration of renewable energy sources. The interaction between oil shale and biomass increases the concentrations of CO and CH4 in the producer gas. Pine bark exhibits the highest CO concentration and the blends of pine bark char have the lowest surface area among the tested samples.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Lin Chen, Di Wu, Guanghao Chen
Summary: Sodium ions have inhibitory effects on the sulfate reagent in the BSP assay, while the presence of potassium ions can enhance the anaerobic biodegradability of organic substrates.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Guiyin Wang, Ruoqi Liu, Zhanbiao Yang, Wei Liu, Xiaoxun Xu, Shirong Zhang, Zhang Cheng, Guochun Lv, Changlian Xu, Junzhuo Cai, Zhien Pu
Summary: Magnetic magnesium-loaded Chinese herbal medicine residues (MM-TCMRs) were prepared for simultaneous removal and recovery of phosphate and ammonium from wastewater. The MM-TCMRs exhibited larger surface areas and rougher structures, and could be separated by adjusting a magnetic field. The adsorption of phosphate and ammonium by MM-TCMRs followed the pseudo-second-order model, with high maximum adsorption capacities. Furthermore, the MM-TCMRs were found to positively impact soil fertility and alfalfa growth.
BIORESOURCE TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Carmen Sanchez, Sonia Garde, Jose Maria Landete, Javier Calzada, Dave J. Baker, Rhiannon Evans, Arjan Narbad, Melinda J. Mayer, Marta Avila
Summary: This study sequenced and compared the genomes of bacteriophages active against C. tyrobutyricum, identifying novel species and phage proteins. A phage showed high lytic activity against C. tyrobutyricum cells and remained stable under different environmental conditions. Additionally, engineered strains successfully delivered endolysin and fluorescent protein to dairy products without affecting their technological properties.
Article
Biotechnology & Applied Microbiology
Danai Etter, Michael Biggel, Mariella Greutmann, Nicole Cernela, Sophia Johler
Summary: Bacillus cytotoxicus is a thermotolerant bacterium that is linked to rare but sometimes fatal cases of diarrheal disease. It is mostly found in dehydrated foods containing potato starch or insects and may be missed in routine screening.
Article
Biochemical Research Methods
Arnaud Marchant, Pierre Van Damme, Stanley Plotkin, Pieter Neels, Maria Cristina Cassetti, Jakob Cramer, Marion F. Gruber, David Goldblatt, Deborah King, Wendy Hartig-Merkel, Joris Vandeputte
Summary: A meeting was held in February 2023 by international organizations to discuss correlates of protection against COVID-19, reviewing evidence and identifying knowledge gaps. Neutralizing antibodies and T lymphocytes are believed to play a significant role in protection, but further studies are needed to consolidate the evidence and evaluate other potential factors.
Article
Agricultural Engineering
Juan Wang, Minna Jiao, Xiangyu Zhan, Cuihuan Hu, Zengqiang Zhang
Summary: This study found that the combined use of a semi-permeable membrane and mature compost in pig manure composting can increase the concentration of humic substances, promote fungal community succession, and adding 15% MC under membrane covering can optimize the quality of composting products.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Runhua Su, Huimin Fu, Lili Ding, Bo Fu, Su He, Haijun Ma, Haidong Hu, Hongqiang Ren
Summary: This study investigated the effects of long-term and continuous addition of NZVI on methanogenic activity, microbial community, and transcription activity. The results showed that low levels of NZVI inhibited methanogenesis, while high levels led to a sharp decrease in methane production and chemical oxygen demand removal. NZVI had adverse effects on cell viability and enzyme activity.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Lin Zhu, Yuxiang Zhao, Siyin Chen, Xinyin Miao, Zhou Fang, Xiangwu Yao, Chifei Dong, Baolan Hu
Summary: The study found that alternating ventilation can stabilize moisture content, reduce temperature and oxygen concentration range. It extended the duration of high temperature, improved the cooperation intensity of microbes, and stimulated the growth of core microbes. Additionally, it improved the seed germination index and humic acid content of the compost.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Ragavan Chandrasekar, Mohammed Askkar Deen, Selvaraju Narayanasamy
Summary: In this study, catalytic hydrothermal carbonization was used to synthesize hydrochar as an alternative to pyrochar for the elimination of emerging contaminants in multicomponent systems. The synthesized hydrochar showed high specific surface area and excellent adsorption efficiency, making it a potential candidate for the removal of emerging contaminants.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Jia Yun Chang, Taufiq Ahmad Syauqi, Kumar Sudesh, Si Ling Ng
Summary: Polyhydroxyalkanoates (PHAs) serve as effective biocarriers for biofilm development, showing excellent attachment performance and stable biofilm structure in acid orange 7 treatment.
BIORESOURCE TECHNOLOGY
(2024)
