Article
Biophysics
Xiaojie Qin, Binqi Wei, Yuanhang Xiang, Hao Lu, Fengfei Liu, Xinchun Li, Fan Yang
Summary: This study utilizes the coordination between exosomes and metal-organic frameworks to develop an ultrasensitive detection method for tumor-derived exosomes. The method is highly specific and fast, allowing for the profiling of different cancer types and accurate diagnosis in a clinical setting. This strategy provides new opportunities for functional materials assembly and precision diagnostics.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Bo Peng, Xiaoxue Liu, Yao Yao, Jianfeng Ping, Yibin Ying
Summary: This study proposed wearable electrodes for real-time monitoring of leaf capacitance, which showed good flexibility and stability. The capacitance value was found to be positively correlated with leaf moisture content and showed higher value at night compared to daytime. The growth and physiological parameters of the plant were not significantly affected during capacitance monitoring using the wearable electrodes. These results demonstrate the potential of wearable electrodes for real-time and precise monitoring of plant physiological information in the future.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Xinyu Liu, Xiaoming Su, Mingyang Chen, Yangcenzi Xie, Ming Li
Summary: Rapid early diagnosis of Alzheimer's disease is crucial, but challenging. In this study, a self-calibrating biosensor based on SERS-LFIA technique is developed for accurate AD diagnosis. It has high sensitivity, excellent anti-interference capability, lowcost and easy operation.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Xiaole Han, Hongyan Yu, Li Zhang, Zhi Weng, Ling Dai, Li Wang, Lin Song, Zhongzhong Wang, Rong Zhao, Luojia Wang, Weitao Wang, Dan Bai, Yongcan Guo, Ke Lv, Guoming Xie
Summary: This study proposes a movable toehold principle to suppress leakage and accelerate catalytic reactions. The catalytic hairpin assembly with movable toehold exhibits a good signal-to-background ratio, robustness, and specificity, and demonstrates satisfactory performance in complex circuits.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Shunsuke Rokutani, Kentaro Hiraka, Hiroshi Saitoh, Taiki Saito, Yoshihiko Nonaka, Kinuko Ueno, Kaori Tsukakoshi, Noriyuki Ohnishi, Kazunori Ikebukuro
Summary: In this study, a DNA aptamer-based thermo-responsive magnetic particle (TM) assay was developed as a simple and sensitive protein detection system. The research demonstrated that the modification of aptamers on TM successfully controlled the aggregation behavior and enabled the detection of target proteins. By using capturable aptamers, the sensitivity and limit of detection of the TM assay were improved.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biotechnology & Applied Microbiology
Mariana Marggraf, Andrea Barac, Jens Andre Hammerl, Stefan Hertwig
Summary: Isolating Yersinia enterocolitica from food is challenging due to overgrowth by other microflora and the lack of a selective medium. The authors improved the standard isolation procedure, resulting in a more sensitive method, reduced background bacteria, and time savings.
Article
Biotechnology & Applied Microbiology
Rafael D. Chaves, Samuel H. Kumazawa, Amin Mousavi Khaneghah, Veronica O. Alvarenga, Humberto M. Hungaro, Anderson S. Sant'Ana
Summary: This study investigated the susceptibility to sanitizers and biofilm-forming ability of 43 Salmonella enterica and Listeria monocytogenes strains on stainless steel surfaces. The results showed variations in susceptibility to sanitizers and biofilm resistance among different strains of the pathogens, with chlorine compounds being more effective in inactivating planktonic cells and biofilms.
Article
Biotechnology & Applied Microbiology
Yuan Rao, Wei Zhuang, Jinle Liu, Ting Tang, Zhi Wang, Hanjie Ying
Summary: This study describes a mild and versatile strategy to immobilize glucose oxidase (GOD) on carboxyl-activated metal-organic framework (MOF) through DNA-directed immobilization technology. The immobilized GOD showed enhanced stability and catalytic activity, making it a potential candidate for applications in biocatalysis and biotechnology.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Agricultural Engineering
Pedram Karimipour-Fard, Chonlong Chio, Alyssa Brunone, Heera Marway, Michael Thompson, Niloofar Abdehagh, Wensheng Qin, Trent Chunzhong Yang
Summary: In this study, a high-solids twin-screw extrusion approach was developed to increase the biomethane production from forestry lignocellulosic waste by up to 190%. This finding provides a promising method for industrializing biomethane production.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Yafei Wei, Yimeng Jiao, Hongbo Chen
Summary: This study investigates the effect of polydimethyldiallylammonium chloride (PDDA) on the dark fermentation process of sludge and reveals that PDDA inhibits hydrogen production by reducing the activity of key enzymes and enriching other microorganisms, thus impeding the hydrogen production via the acetate pathway. The findings deepen the understanding of the potential effects of PDDA on sludge treatment and provide a theoretical basis for mitigating the negative impacts of quaternary ammonium-based cationic flocculants.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Huanyu Bao, Zhiqiang Chen, Qinxue Wen, Yiqi Wu, Qiqi Fu
Summary: This study investigated the impact of oxytetracycline on antibiotic resistance genes in soil and revealed the mechanism of gene dissemination during composting. The findings showed that oxytetracycline increased the abundance of resistance genes and highlighted the importance of countermeasures to mitigate potential hazards.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiaoxiao Li, Xinxin Liu, Junren Zhang, Fu Chen, Muhammad Khalid, Jieqi Ye, Martin Romantschuk, Nan Hui
Summary: This research investigated the degradation properties of polyethylene terephthalate (PET) by PET hydrolase (WCCG) in high-temperature composting and its impact on microbial communities. The study found that the addition of WCCG enzyme slowed down the degradation of PET and reduced the abundance of thermophilic bacteria. However, potential PET degrading microbiota, such as Acinetobacter and Bacillus, were enriched in the composters where PET was reduced without WCCG addition. The external introduction of the WCCG enzyme to the compost reacted with PET and inhibited the proliferation of plastic-degrading bacteria, resulting in a 35% degradation of PET. The research suggests that both the WCCG enzyme and the microbiota associated with plastic-degradation have the potential to reduce PET, providing a novel method for mitigating pollution caused by environmental microplastics.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Yumi Uruno, Juwon Lee, Hanseob Jeong, Jaewon Chung
Summary: This study investigated the particle behavior in a Y-junction mixer and found that a vertically oriented mixer with an upward-directed supercritical water inlet achieved the most effective mixing, leading to improved hydrolysis efficiency. This configuration also minimized backflow.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Xiaonong Zhang, Naif Abdullah Al-Dhabi, Bo Gao, Li Zhou, Xingxing Zhang, Zixuan Zhu, Wangwang Tang, Amen Chuma, Chongjun Chen, Peng Wu
Summary: This article investigates the buffering capacity and recovery-enhancing ability of granular activated carbon (GAC) in a starved anaerobic ammonium oxidation (anammox) reactor. The findings reveal that GAC's surface characteristics enable faster recovery and improved nitrogen removal efficiency. Additionally, GAC promotes extracellular polymeric substance secretion, bacterial proliferation, and microbial community restoration. Metabolic function analysis demonstrates that GAC stimulates more diverse metabolic networks.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Yaqing Liu, Si Tang, Qi Yan, Jin Zhou, Zhonghua Cai
Summary: This study investigated the effectiveness and mechanisms of biofilm attached cultivation (BAC) under mixotrophy in promoting algal proliferation. The results showed that BAC significantly increased the biomass productivity of the algae compared to suspended cultivation. The findings suggest that the immobilized lifestyle in BAC leads to optimized growth and production of commercially valuable algal species.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Kaiyi Wu, Tino Leliveld, Hans Zweers, Huub Rijnaarts, Alette Langenhoff, Tania Fernandes
Summary: Single microalgae species are effective at removing various organic micropollutants, but increased species diversity may enhance this removal. In this study, a mixed microbial community showed higher removal capacity than Chlorella sorokiniana at a specific stage, but removal efficiency decreased due to overgrowth of filamentous green algae.
BIORESOURCE TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Guanghui Zhou, Pengcheng Dong, Xin Luo, Lixian Zhu, Yanwei Mao, Yunge Liu, Yimin Zhang
Summary: Interactions between bacterial species in the formation of dual-species biofilms in the food industry are affected by environmental stresses, such as temperature and pH. This study found that the combination of cold and acid stresses significantly increased biofilm formation, and different temperatures resulted in variations in the presence of specific bacterial species and the secretion of extracellular substances. The findings provide valuable insights for addressing mixed biofilms in food processing under diverse stressful conditions.
