Article
Biophysics
Chunlian Qin, Qunchen Yuan, Mengxue Liu, Liujing Zhuang, Lizhou Xu, Ping Wang
Summary: This study proposes a biohybrid tongue based on hypothalamic neuronal network coupled with microelectrode array for real-time glucose perception. Hypothalamic neuronal cultures were cultivated on a two-dimensional brain-on-chip device, enabling the formation of neuronal networks and electrophysiological signal detection. The study investigates the endogenous expression of sweet taste receptors in hypothalamic neuronal cells and explores the spike signal response to sucrose and glucose stimulation. The biohybrid tongue provides insights into glucose sensing mechanisms and hypothalamic neuronal function.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Edward J. Jacobs, Kenneth N. Aycock, Pedro P. Santos, Joanne L. Tuohy, Rafael V. Davalos
Summary: The efficiency of electroporation treatments depends on the application of a critical electric field over the targeted tissue volume. A deep neural network trained on finite element model simulations was developed to predict tissue properties from a measured voltage ramp, showing minimal test error and great utility in clinical applications. The platform can quickly ascertain patient-specific tissue properties required for treatment planning.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Qi Xu, Jinze Li, Zhiqi Zhang, Qi Yang, Wei Zhang, Jia Yao, Yaxin Zhang, Yueye Zhang, Zhen Guo, Chao Li, Shuli Li, Changsong Zhang, Chuanxin Wang, Lutao Du, Chuanyu Li, Lianqun Zhou
Summary: Real-time digital polymerase chain reaction (qdPCR) integrates absolute quantification with process information, improving precision in molecular diagnostics. We propose a novel approach to determine optimal reaction conditions for qdPCR using real-time fluorescence monitoring in microwells. By leveraging an ultrathin laminated chip (UTL chip), we achieve faster amplification and higher accuracy, showing promise for molecular diagnostics.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
M. Mahabubur Rahman, Nabil H. Bhuiyan, Minjun Park, M. Jalal Uddin, Gyeong J. Jin, Joon S. Shim
Summary: In this work, a novel and low-cost fabrication technology for interdigitated electrode array (IDA) electrodes on the polymer substrate was developed. The fabricated IDA electrode showed high sensitivity and detection limit in electrochemical detection of biomarkers.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Jun Wang, Jiawen Han, Juan Wang, Xujuan Lv, Daoqing Fan, Shaojun Dong
Summary: This study reports a ratiometric fluorescent platform based on nanozymes for the highly sensitive detection of cysteine and bleomycin. The platform exhibits acceptable performance and high selectivity, and shows good performance in human serum samples. Additionally, DNA contrary logic pairs and multilevel concatenated circuits were fabricated, enriching the building blocks of biocomputing.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Yinru Zhu, Benqing Wu, Xinwei Gao, Yong Guo, Xiao Peng, Luwei Wang, Xuewu Tian, Jian Wang, Wei Yan, Hui Zhang, Junle Qu
Summary: Preeclampsia is a common pregnancy complication and the leading cause of maternal and perinatal mortality. However, early diagnostic methods for preeclampsia are still rare. This study explores the possibility of using fluorescence lifetime imaging and the Nile Blue probe for the early diagnosis and severity prediction of preeclampsia.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Bettina Glahn-Martinez, Beatriz Jurado-Sanchez, Elena Benito-Pena, Alberto Escarpa, Maria C. Moreno-Bondi
Summary: This study developed a novel bioassay for monitoring FK506 using magnetic Janus micromotors and a recombinant chimera receptor. The bioassay utilized a fluorescence competitive method between the drug and the micromotors to achieve a one-step assay without washing steps. The method exhibited a high limit of detection and dynamic range, and was able to operate in raw samples.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Maria Reynoso, An-Yi Chang, Yao Wu, Raygan Murray, Smrithi Suresh, Yuma Dugas, Joseph Wang, Netzahualcoyotl Arroyo-Curras
Summary: Molecular monitoring in the dermal interstitial fluid (ISF) has the potential to screen markers of health and disease status in a painless manner. This study presents an innovative approach to address the poor patch stability of microneedle sensors on live rodents by using magnetic placement. Real-time pharmacokinetic measurements in live rats demonstrate the effectiveness of this approach. The future prospects and challenges of this technology are also discussed.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Vo Thi Nhat Linh, Hongyoon Kim, Min-Young Lee, Jungho Mun, Yeseul Kim, Byeong-Ho Jeong, Sung-Gyu Park, Dong-Ho Kim, Junsuk Rho, Ho Sang Jung
Summary: This study developed a flexible and highly absorptive three-dimensional plasmonic nanostructure sensor for rapid lung cancer screening and diagnosis. The sensor successfully detected and classified lung cancer in patient saliva samples. Additionally, the study investigated important Raman peak positions related to different stages of lung cancer, providing insights for early-stage cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kaijing Wang, Congcong Zhang, Han Zhou, Hongshuai Wei, Liping Yin, Tingting Zhang, Yunqing Zhi, Jun Zhou, Bingkai Han, Zhenguo Zhang, Xin Du
Summary: In this study, an electrochemical platform for detecting GLUT-1 on living cells was developed using a composite of reduced graphene oxide-multi-wall carbon nanotube. The sensor demonstrated good performance in detecting GLUT-1 expression on cells and showed consistency with traditional detection methods. It has the potential to assess malignancy level and glucose uptake pathways in tumor cells while reducing medical costs.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Haojie Jin, Jiaqi Zhang, Yan Wang, Wanzhao Ge, Yike Jing, Xiaoyu Cao, Yixin Huo, Yujie Fu
Summary: In this study, a live-cell bio-monitoring system was proposed to assess phenylalanine bioavailability in real time in cyanobacteria based on codon de-generacy and species-specific usage bias. Increasing intracellular phenylalanine bioavailability could promote cell growth and provide a screening method for phenylalanine content.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Anna Klebes, H. Ceren Ates, Rene D. Verboket, Gerald A. Urban, Felix von Stetten, Can Dincer, Susanna M. Frueh
Summary: This review discusses the development and application of multianalyte biosensors. Simultaneous detection of protein and nucleic acid biomarkers is crucial for improving diagnostic accuracy and therapeutic success. The review also highlights the importance of multianalyte analysis in the field of single-cell multiomics and discusses the potential of multianalyte biosensors in medical diagnostics.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biochemistry & Molecular Biology
Kazufumi Ohmura, Takamasa Kinoshita, Hiroyuki Tomita, Hideshi Okada, Masayoshi Shimizu, Kosuke Mori, Toshiaki Taniguchi, Akio Suzuki, Toru Iwama, Akira Hara
Summary: The study found that Vincristine-induced peripheral neuropathy can be improved by protecting the endothelial glycocalyx. The results suggest that nafamostat may be a potential new drug for the treatment of VIPN.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Jaw Long Sun, Jae Hak Ryu, Wonjun Cho, Heeseung Oh, A. M. Abd El-Aty, Didem Ozkal Eminoglu, Ji Hoon Jeong, Tae Woo Jung
Summary: The study aimed to investigate the impact of CTRP4 on inflammation and the interaction between endothelial cells and monocytes under hyperlipidemic conditions. The results showed that CTRP4 reduced the attachment of THP-1 monocytes to HUVECs and decreased inflammation indicators in experimental cells exposed to hyperlipidemic conditions. Additionally, CTRP4 improved the interaction between endothelial cells and monocytes through the SIRT6/Nrf2-dependent pathway.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
