Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Bo Peng, Xiaoxue Liu, Yao Yao, Jianfeng Ping, Yibin Ying
Summary: This study proposed wearable electrodes for real-time monitoring of leaf capacitance, which showed good flexibility and stability. The capacitance value was found to be positively correlated with leaf moisture content and showed higher value at night compared to daytime. The growth and physiological parameters of the plant were not significantly affected during capacitance monitoring using the wearable electrodes. These results demonstrate the potential of wearable electrodes for real-time and precise monitoring of plant physiological information in the future.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Xinyu Liu, Xiaoming Su, Mingyang Chen, Yangcenzi Xie, Ming Li
Summary: Rapid early diagnosis of Alzheimer's disease is crucial, but challenging. In this study, a self-calibrating biosensor based on SERS-LFIA technique is developed for accurate AD diagnosis. It has high sensitivity, excellent anti-interference capability, lowcost and easy operation.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Xiaole Han, Hongyan Yu, Li Zhang, Zhi Weng, Ling Dai, Li Wang, Lin Song, Zhongzhong Wang, Rong Zhao, Luojia Wang, Weitao Wang, Dan Bai, Yongcan Guo, Ke Lv, Guoming Xie
Summary: This study proposes a movable toehold principle to suppress leakage and accelerate catalytic reactions. The catalytic hairpin assembly with movable toehold exhibits a good signal-to-background ratio, robustness, and specificity, and demonstrates satisfactory performance in complex circuits.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Shunsuke Rokutani, Kentaro Hiraka, Hiroshi Saitoh, Taiki Saito, Yoshihiko Nonaka, Kinuko Ueno, Kaori Tsukakoshi, Noriyuki Ohnishi, Kazunori Ikebukuro
Summary: In this study, a DNA aptamer-based thermo-responsive magnetic particle (TM) assay was developed as a simple and sensitive protein detection system. The research demonstrated that the modification of aptamers on TM successfully controlled the aggregation behavior and enabled the detection of target proteins. By using capturable aptamers, the sensitivity and limit of detection of the TM assay were improved.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biochemistry & Molecular Biology
M. Paul Murphy, Valeria A. Buzinova, Carrie E. Johnson
Summary: Progress has been made in the treatment of Alzheimer's disease through the development of anti-A beta therapeutics, which have shown modest efficacy in slowing the progression of the disease. However, the puzzling issue remains as to why completely removing A beta does not fully stop the disease.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Neha Sawant, Sudhir Kshirsagar, P. Hemachandra Reddy, Arubala P. Reddy
Summary: Depression is a common neuropsychiatric comorbidity in Alzheimer's disease (AD) and other Tauopathies. Selective serotonin reuptake inhibitor (SSRI) treatment, such as Citalopram, not only has anti-depressive and anxiolytic effects, but also helps improve neurogenesis, reduce amyloid burden & Tau pathologies, and neuroinflammation in AD. In this study, Citalopram was found to reduce pathologically pTau level, increase synaptic gene expression and cytoskeletal structure, as well as improve cell survival, mitochondrial respiration, and mitochondrial morphology in cells expressing mutant APP and Tau. These findings suggest that Citalopram could be a promising therapeutic drug for treating depression and AD.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Alexander Koerner, Andre Bazzone, Maximilian Wichert, Maria Barthmes, Srujan Kumar Dondapati, Niels Fertig, Stefan Kubick
Summary: In this study, the human Peptide Transporter 1 (hPepT1) and its interactions with various substrates were comprehensively studied using solid supported membrane-based electrophysiology (SSME). The pH dependence of hPepT1 activity was observed, and the K-M and relative V-max values of different substrates were determined. Specific inhibition of hPepT1 by various inhibitors was also evaluated in a high throughput format.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Sandra Tejerina-Miranda, Maria Pedrero, Marina Blazquez-Garcia, Veronica Serafin, Ana Montero-Calle, Maria Garranzo-Asensio, A. Julio Reviejo, Jose M. Pingarron, Rodrigo Barderas, Susana Campuzano
Summary: This study developed the first electrochemical bioplatform for determining human endostatin, which shows high sensitivity and selectivity for diagnosing tumors and identifying patients in different stages.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Andrea Fasano, Vincent Fourmond, Christophe Leger
Summary: This article focuses on the electrochemical response when a redox enzyme or synthetic catalyst is interfaced with an electrode, and the relation to the catalytic cycle and properties of the catalytic intermediates. The concept of catalytic pKa is introduced, and understanding and tuning catalytic potentials are crucial for the design of reversible catalysts that operate at a fast rate in response to even a small overpotential.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Delun Zheng, Ruilong Zhang, Kaibo Zheng, Caiyun Zhang, Jianqiao Chen, Chengwen Wang, Shaochen Sun, Sihan Lin
Summary: This study successfully prepared a high-performance biosensor with superior photoelectrochemical properties by fabricating a contact heterojunction interface with special porosity structure.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Edward J. Jacobs, Sabrina N. Campelo, Alyssa Charlton, Sara Altreuter, Rafael V. Davalos
Summary: The relationships between burst number, reversible, irreversible, and calcium electroporation in tumor tissue-mimics have been comprehensively evaluated in this study. The findings show that electroporation effects saturate with an increasing burst number, and the reversible and irreversible electroporation thresholds converge at a certain point. The lethal thresholds for calcium electroporation are similar to reversible electroporation. The study emphasizes the importance of considering burst number in minimizing thermal damage, choosing adjuvant therapies, and modeling electroporation effects at low burst numbers.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Shuai Xu, Lina Kong, Lin Li, Changyuan Wang, Jiangning Gu, Haifeng Luo, Qiang Meng
Summary: This study found that FXR is significantly reduced in the livers of NAFLD patients. Overexpression of FXR can improve NAFLD and inhibit oxidative stress and AIM2 inflammasome activation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Humayun Amir, Vasanth Subramanian, Sundaram Sornambikai, Nagamony Ponpandian, Chinnusamy Viswanathan
Summary: In this study, the electrochemical detection of breast cancer biomarker HER2 was successfully achieved using the N-CQDs/GS as the detector. The BSA-modified HER2 Antibody/N-CQDs/GS immunoelectrode preserved excellent activity for the biosensor, while the GS electrode provided high stability and conductivity. The method exhibited a low detection limit and optimal specificity, stability, and reproducibility for detecting HER-2 protein in untreated blood samples from breast cancer patients.
BIOELECTROCHEMISTRY
(2024)
Review
Biochemistry & Molecular Biology
Hui Chen, Cheng Jin, Li Xie, Jian Wu
Summary: Succinate, an intermediate of the TCA cycle, is crucial in mitochondrial metabolism, energy production, and is also considered a signaling molecule in metabolism and hepatic diseases. Its downstream signaling pathway through GPR91 leads to various intracellular responses, including succinylation, ROS production, HIF-1 alpha stabilization, and significant impact in cellular metabolism due to its role in the TCA cycle. Understanding the signaling mechanisms of succinate in hepatic fibrosis, metabolic reprogramming, inflammatory or immune responses, and carcinogenesis is of great interest. This review aims to provide the current understanding of succinate in mediating metabolism, inflammatory and immune responses in liver diseases, with the aim of establishing a molecular basis for therapeutic strategies.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Divya Sharma, Puneet Rawat, Victor Greiff, Vani Janakiraman, M. Michael Gromiha
Summary: COVID-19 has caused millions of deaths and had a severe impact on global economies. The emergence of SARS-CoV-2 variants has posed significant challenges in controlling the pandemic, particularly in terms of their potential to evade the immune system and vaccine immunity. In this study, a machine learning model was developed to predict the ability of mutations in the SARS-CoV-2 spike protein to reduce the neutralizing ability of antibodies. The model achieved a high level of accuracy in distinguishing high or low escape mutations. The findings showed that high escape mutations were dominated by charged to nonpolar mutations, while low escape mutations were dominated by polar to nonpolar mutations. This method can aid in prioritizing high/low escape mutations and assist in antibody design for current and emerging variants targeting the SARS-CoV-2 RBD region.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Hang Lam Li, Simei Go, Jung-Chin Chang, Arthur Verhoeven, Ronald Oude Elferink
Summary: This review highlights the distinct characteristics and crucial role of soluble adenylyl cyclase (sAC) in cellular processes, as well as recent significant advancements in the field of sAC research.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biophysics
Sengwang Fu, Jianping Zhu, Zhijun Jiang, Yue Cao, Yufei Chen, Lihao Zhang, Sunlong Li, Weipeng Lu, Chengbin Miao, Qing He, Qi Li, Weixing Zhang, Lehao Ren, Yachun Li, Hongchao Shi, Cihui Liu
Summary: Effective management of biofluids is crucial for in vivo surgical interventions. Recent advances include self-sealing needles, drug-eluting stents, and shear-thinning hydrogels. However, complications associated with intestinal mucosal injury and secondary damage still persist. In this study, researchers developed an interpenetrating Janus wettability stent coating that enables unidirectional draining of excessive biofluid. They also demonstrated directional biofluid movement using a self-pumping dressing with potential applications in biofluid collection and disease diagnosis through metal ion detection. This integrated system presents an opportunity for designing wound dressings with effective biofluid management and metal ion detection capabilities.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2024)
