Agriculture, Dairy & Animal Science

Article Agriculture, Dairy & Animal Science

Oxford nanopore sequencing as an alternative to reduced representation bisulphite sequencing for the identification of CpGs of interest in livestock populations

Adrian Lopez-Catalina, Valentin Costes, Ramon Peiro-Pastor, Helene Kiefer, Oscar Gonzalez-Recio

Summary: Epigenetic marks can explain part of the phenotypic variance in animal breeding models. Nanopore sequencing shows high correlation with bisulphite sequencing in identifying DNA methylation marks. Different sequencing methods highlight differentially methylated genes and positions of economic interest in animal breeding.

LIVESTOCK SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Can steam be usable as a plus for ventilation shutdown?

A. V. Mendoza, S. Weimer, Z. Williams

Summary: This study evaluated the effectiveness of steam as an additional method for depopulation of laying hens in a cage-free aviary system. The results showed that steam alone or in combination with heat can lead to faster mortality of hens, and the distribution of carcasses varies in different tiers.

JOURNAL OF APPLIED POULTRY RESEARCH (2024)

Article Agriculture, Dairy & Animal Science

Research Note: Transcriptome analysis of skeletal muscles of black-boned chickens, including 2 types (wild and mutated) of Taihe black-boned silky fowl and 1 type (wild) of Yugan black-boned chicken

Cong Huang, Yue Wei, Zhaofeng Kang, Weihong Zhang, Yanping Wu

Summary: This study identifies candidate genes and pathways responsible for melanogenesis in Taihe black-boned silky fowl through digital gene expression tag-based transcriptome analysis. It also finds that muscle-related and signaling-related biological processes play important roles in melanogenesis.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Association of vitamin D status with redox balance and insulin resistance and its predicting ability for subclinical pregnancy toxemia in pregnant sheep

Mehak Nisar, Shafayat Ahmad Beigh, Abdul Qayoom Mir, Syed Ashaq Hussain, Aijaz Ahmad Dar, Iqbal Yatoo, Adil Mehraj Khan

Summary: This study aimed to evaluate the role of vitamin D in redox balance, insulin resistance, and its predicting value for subclinical pregnancy toxemia (SPT) in pregnant ewes. The results showed that ewes with insufficient vitamin D had significantly lower total antioxidant capacity (TAC) compared to ewes with sufficient vitamin D, and there were significant differences in metabolite concentrations between the two groups. Additionally, vitamin D was positively correlated with fructosamine, calcium, and TAC, and negatively correlated with non-esterified fatty acid (NEFA) and total oxidant status (TOS). Ewes with insufficient vitamin D had a higher risk of developing SPT two weeks prior to lambing and at lambing.

DOMESTIC ANIMAL ENDOCRINOLOGY (2024)

Article Agriculture, Dairy & Animal Science

Use of an elevated platform with perforated surface and manure belt by fast- growing broilers on commercial farms

F. May, B. Spindler, J. Muesse, K. Skiba, N. Kemper, J. Stracke

Summary: This study evaluated the use of an elevated platform with perforated surface and manure belt by fast-growing broilers. The results showed that broilers were able to effectively use the platform and ramp for resting throughout different stages of growth. Factors such as a shallow ramp incline, wide ramps, and appropriate materials contributed to the high utilization of the platform. Further research is needed to improve the platform design for undisturbed resting of broilers.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Nanoemulsion-based plant essential oil formulations: in vitro evaluation of pesticidal activity against ectoparasites in poultry

Anuwat Lakyat, Jarongsak Pumnuan, Thanaporn Doungnapa, Sudjai Phutphat, Somsak Kramchote, Kamronwit Thipmanee

Summary: This study assessed the efficacy of nanoemulsion-based plant essential oil formulations from clove, cinnamon, and turmeric essential oils against poultry ectoparasites. The formulations showed exceptional potency in exterminating ectoparasites and demonstrated repellent properties.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Comparative analysis of hepatic transcriptomes and metabolomes of Changshun green-shell laying hens based on different green eggshell color intensities

Wenbin Xu, Ren Mu, Tuya Gegen, Jiaxiang Luo, Yang Xiao, Shunnian Ou, Qi Wu, Yongsong Zuo, Zhi Chen, Fangwei Li

Summary: This study analyzed the hepatic transcriptomes and metabolomes of Changshun green-shell hens laying dark green and light green eggs to investigate the role of the liver in regulating eggshell color. The results suggest that hepatic melanin metabolism and transport are critical for eggshell coloration, and several candidate genes and metabolites were identified to potentially play important roles in this process.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Transcriptome profiling reveals SLC5A5 regulates chicken ovarian follicle granulosa cell proliferation, apoptosis, and steroid hormone synthesis

Xiaoxu Shen, Xiyu Zhao, Haorong He, Yao Zhang, Qing Zhu, Huadong Yin

Summary: SLC5A5 plays a crucial role in the development and function of chicken ovarian follicle granulosa cells, influencing cell proliferation, steroid hormone synthesis, and apoptosis. This discovery sheds light on the broader context of chicken ovarian follicle development and potential ways to improve egg-laying performance through molecular marker-assisted breeding technology.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Colonization of chickens with competitive exclusion products results in extensive differences in metabolite composition in cecal digesta

Lenka Vlasatikova, Michal Zeman, Magdalena Crhanova, Jitka Matiasovicova, Daniela Karasova, Marcela Faldynova, Hana Prikrylova, Alena Sebkova, Ivan Rychlik

Summary: This study compared the metabolites in the cecal digesta of differently colonized chickens and found that competitive exclusion products can affect the metabolite profiles in the chicken cecum, increasing the abundance of nucleotides, amino acids, and vitamins, while decreasing the abundance of compounds derived from plant feed.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Lipidomics reveals lipid changes in the intramuscular fat of geese at different growth stages

Zhi Cao, Maodou Xu, Shangzong Qi, Xinlei Xu, Wei Liu, Linyu Liu, Qiang Bao, Yang Zhang, Qi Xu, Wenming Zhao, Guohong Chen

Summary: This study investigated the lipid composition and metabolic pathways of goose meat, revealing changes in intramuscular fat deposition and lipid composition with age. Glyceride molecules showed significant changes and played a pivotal role in intramuscular fat deposition. Pathway analysis identified glycerophospholipid and glyceride metabolism as the most significant pathways associated with lipid changes during goose growth.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Analysis of progesterone and estrone-sulfate in feces of American Bison using liquid chromatography coupled to mass spectrometry: Technical validation and correlation with blood levels

Patrice Dufour, Vincent Frisee, Goulven Rigaux, Flore Brutinel, Sophie Egyptien, Philippe Bossaert, Jessica Deleersnyder, Stefan Deleuze, Stephanie Peeters, Caroline Le Goff, Jerome Ponthier, Etienne Cavalier

Summary: This report describes the method of measuring progesterone and estrone-sulfate using Liquid Chromatography coupled with Mass Spectrometry in American Bison feces. This method provides an alternative to serum assay for studying the pregnancy-related evolution of these hormones in American Bison.

DOMESTIC ANIMAL ENDOCRINOLOGY (2024)

Article Agriculture, Dairy & Animal Science

Concentration of lipids, cholesterol, and fatty acid profile in chicken breast meat affected by wooden breast myopathy frozen for up to 12 mo

Erick Alonso Villegas-Cayllahua, Daniel Rodrigues Dutra, Rodrigo Fortunato de Oliveira, Mateus Roberto Pereira, Erika Nayara Freire Cavalcanti, Fabio Borba Ferrari, Rodrigo Alves de Souza, Heloisa de Almeida Fidelis, Aline Giampietro-Ganeco, Pedro Alves de Souza, Juliana Lolli Malagoli De Mello, Hirasilva Borba

Summary: This study examined the effects of frozen storage on lipid and cholesterol concentrations, as well as fatty acid distribution, in wooden chicken breast meat. The results showed that wooden breast myopathy led to lower levels of beneficial polyunsaturated fatty acids, such as DHA, EPA, and ARA, in chicken meat. Prolonged storage further impaired the nutritional value of the meat.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Effects of monochromatic light on hepatic glycogen and lipid synthesis in broilers

Lu Wang, Zixu Wang, Yaoxing Chen, Jing Cao

Summary: This study investigated the effects of monochromatic LED lights on liver glycogen and lipid synthesis in broilers. The results showed that liver, abdominal fat, and abdominal fat indices increased with age. Liver glycogen and triglyceride contents also showed an increasing trend. mRNA levels of several genes related to glycogen and lipid synthesis in the liver varied with different light sources and ages. Total antioxidant capacity and liver superoxide dismutase contents increased with age and light source.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Oral bioavailability and egg drug residue of lincomycin in laying hens after different treatment

Jin-Hwa Kim, Je-Won Ko, Jeong-Won Kim, Ji-Soo Jeong, Chang-Yeop Kim, In-Sik Shin, Tae-Won Kim

Summary: This study investigated the oral bioavailability of lincomycin (LCM) and compared drug residue patterns in eggs. The results showed that LCM had a low oral absorption rate in laying hens, which was consistent with the findings in egg residue profiles.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Influence of the genotype of the hen (Gallus gallus domesticus) on main parameters of egg quality, chemical composition of the eggs under uniform environmental conditions

M. Hejdysz, S. Nowaczewski, K. Perz, T. Szablewski, K. Stuper-Szablewska, R. Cegielska-Radziejewska, L. Tomczyk, A. Przybylska-Balcerek, M. Busko, S. A. Kaczmarek, P. Slosarz

Summary: This study aimed to compare the quality characteristics and compounds concentrations in eggs from different breeds and lines of hens. The results showed significant differences in various egg quality parameters, as well as variations in lutein content, vitamin D content, cholesterol content, and fatty acid contents. It was also found that the genetic differences between breeds and lines of hens have a significant impact on the quality of eggs.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Impact of b-glucan dietary supplementation on productive, reproductive performance and physiological response of laying hens under heat stress conditions

Waheed Ezzat, Khalid M. Mahrose, Ahmed M. Rizk, Magdy M. M. Ouda, Ibrahim A. Fathey, Sarah I. Othman, Ahmed A. Allam, Hassan A. Rudayni, Heba A. Almasmoum, Ayman E. Taha, Shatha G. Felemban, Guillermo Tellez-Isaias, Mohamed E. Abd El-Hack

Summary: This study assessed the potential of using B-glucan as an antiheat stress agent for laying hens. The results showed that adding B-glucan improved productive performance, reproductive ability and immune response in hens subjected to heat stress.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Comprehensive evaluation of treating drinking water for laying hens using slightly acidic electrolyzed water

Jian Li, Yang Wang, Weichao Zheng, Tong Xia, Xiangbing Kong, Zhengdong Yuan, Binglong Niu, Guowen Wei, Baoming Li

Summary: This study evaluated two disinfection methods for waterline cleaning in poultry houses and found that using slightly acidic electrolyzed water (SAEW) effectively inhibited bacterial growth with sustained antimicrobial effects. Compared to the traditional high-concentration chemical disinfectant method, SAEW method showed higher fecal normal rate, lower water footprint, and slight improvement in egg quality.

POULTRY SCIENCE (2024)

Article Agriculture, Dairy & Animal Science

Net energy content of hybrid rye fed to growing pigs and effects of particle size on energy values

J. Song, D. M. Holanda, C. H. Heuer, C. M. Nyachoti

Summary: This study determined the energy contents of hybrid rye fed to growing pigs, and investigated the effects of particle size on energy values. Results showed that finely ground hybrid rye had higher digestible energy and metabolizable energy compared to coarsely ground hybrid rye, while net energy only showed a slight improvement.

ANIMAL FEED SCIENCE AND TECHNOLOGY (2024)

Article Agriculture, Dairy & Animal Science

Changes of the salivary and serum proteome in canine hypothyroidism

L. G. Gonzalez-Arostegui, A. Munoz-Prieto, C. P. Rubio, J. J. Ceron, L. Bernal, I. Rubic, V. Mrljak, J. C. Gonzalez-Sanchezc, A. Tvarijonaviciute

Summary: This study investigated changes in the saliva and serum proteome of dogs with hypothyroidism using TMT labelling and LC-MS/MS. The findings showed that this disease induces alterations in the saliva and serum proteome, reflecting physiological and pathological changes in the immune system, metabolism, skin, and coagulation. The downregulation of LDH in saliva may serve as a potential biomarker for canine hypothyroidism.

DOMESTIC ANIMAL ENDOCRINOLOGY (2024)

Article Agriculture, Dairy & Animal Science

Reproductive performance in gilts submitted to non-steroidal therapies to prolong the luteal phase of the estrous cycle

Camila R. C. Brito, Agatha D. Cordeiro, Pricila Baldessar, Carolini Schultz, Monike Quirino, Rafael R. Ulguim, Paulo B. D. Goncalves, Thomaz Lucia Jr, Ivan Bianchi, Bernardo G. Gasperin

Summary: This study tested synchronization protocols without progestogens during the luteal phase in gilts. The results showed that the protocols based on PGF and hCG were effective in reducing the interval between estrus in gilts. Additionally, the hCG2 protocol had negative effects on the number of piglets born alive and total litter size.

DOMESTIC ANIMAL ENDOCRINOLOGY (2024)