期刊
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
卷 97, 期 9, 页码 1295-1303出版社
FERRATA STORTI FOUNDATION
DOI: 10.3324/haematol.2011.058529
关键词
NKG2D; microRNA-1245; TGF-beta 1
类别
资金
- Ministry of Health, Labor and Welfare of Japan
- Ministry of Education, Culture, Sports and Technology of Japan
- Mitani Research and Development Assistance Organization (Kanazawa, Japan)
- Japan Leukemia Research Fund (Tokyo, Japan)
- Hokkoku Gan Kikin Fund (Kanazawa, Japan)
- Grants-in-Aid for Scientific Research [24591418, 21591236] Funding Source: KAKEN
Background NKG2D is an activating receptor expressed by natural killer and T cells, which have crucial functions in tumor and microbial immunosurveillance. Several cytokines have been identified as modulators of NKG2D receptor expression. However, little is known about NKG2D gene regulation. In this study, we found that microRNA 1245 attenuated the expression of NKG2D in natural killer cells. Design and Methods We investigated the potential interactions between the 3'-untranslated region of the NKG2D gene and microRNA as well as their functional roles in the regulation of NKG2D expression and cytotoxicity in natural killer cells. Results Transforming growth factor-beta 1, a major negative regulator of NKG2D expression, post-transcriptionally up-regulated mature microRNA-1245 expression, thus down-regulating NKG2D expression and impairing NKG2D-mediated immune responses in natural killer cells. Conversely, microRNA-1245 down-regulation significantly increased the expression of NKG2D expression in natural killer cells, resulting in more efficient NKG2D-mediated cytotoxicity. Conclusions These results reveal a novel NKG2D regulatory pathway mediated by microRNA-1245, which may represent one of the mechanisms used by transforming growth factor-beta 1 to attenuate NKG2D expression in natural killer cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据