期刊
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
卷 96, 期 5, 页码 779-783出版社
FERRATA STORTI FOUNDATION
DOI: 10.3324/haematol.2010.035931
关键词
cell line; leukemia; thymocyte; interleukin; mouse
类别
资金
- K.U. Leuven
- FWO-Vlaanderen [G.0287.07]
- Foundation against Cancer [SCIE2006-34]
- ERC
The mouse pro-B cell line Ba/F3 has gained major interest as a model system to investigate oncogenic tyrosine kinases and to determine the efficacy of kinase inhibitors. While Ba/F3 cells are suitable to study oncogenic kinases derived from various cell types, the signaling networks in Ba/F3 cells are B-cell specific. We have established a mouse CD4+CD8+ double positive T-cell line (named MOHITO, for MOuse Hematopoietic Interleukin-dependent cell line of T-cell Origin) that has many features of human T-cell acute lymphoblastic leukemia (Notch1 and Jak1 mutation, TCR rearrangement) and is dependent on interleukin-7. The MOHITO cell line can be transformed to cytokine independent proliferation by BCR-ABL1 or mutant JAK1. This mouse T-cell line is a novel model system to investigate protein signaling and inhibition in a T-cell specific context and is a valuable tool to study and verify oncogenic capacity of mutations in the kinome and phosphatome in T-cell malignancies.
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