期刊
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
卷 94, 期 12, 页码 1767-1770出版社
FERRATA STORTI FOUNDATION
DOI: 10.3324/haematol.2009.010900
关键词
flow cytometric diagnosis; acute lymphoblastic leukemia
类别
资金
- Associazione Italiana per la Ricerca sul Cancro (AIRC)
- Milan and Compagnia di San Paolo, Turin
- Fondazione per le Biotecnologie, Turin
Tyrosine kinase inhibitors have profoundly modified the treatment and prognosis of chronic myeloid leukemia and Ph+ acute lymphoblastic leukemia. A rapid and accurate detection of the BCR-ABL fusion protein is paramount today for an optimal management of Ph+ acute lymphoblastic leukemia. We have utilized a recently described and commercialized immunoassay that identifies qualitatively the presence of the BCR-ABL protein in leukemic cell lysates. The BCR-ABL fusion protein is captured and detected by a cytometric bead assay and analyzed by flow cytometry. The assay was applied to 101 primary patient samples (94 acute leukemias and 7 chronic myeloid leukemia blast crisis) and the results of the immunoassay were concordant with those obtained by conventional molecular techniques. The method proved reliable, reproducible, of simple execution and it was successfully completed within four hours. This flow cytometric immunoassay has important implications for perfecting the management of Ph+ acute lymphoblastic leukemia patients worldwide.
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