期刊
GUT
卷 63, 期 8, 页码 1247-1257出版社
BMJ PUBLISHING GROUP
DOI: 10.1136/gutjnl-2012-304213
关键词
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资金
- National Science Fund for Distinguished Young Scholars [81025019]
- National Basic Research Program of China [2012CB517603]
- National Natural Science Foundation of China [J1103512, J1210026, 31070722, 31271013, 31071232, 31170751, 31200695, 51173076, 91129712, 81102489]
- Chinese Ministry of Education [108059]
- PhD Programs Foundation of the Ministry of Education of China [20100091120020]
- Scholarship Award for Excellent Doctoral Student - Ministry of Education
Objective Emerging evidence suggests that microRNA (miRNA)-mediated gene regulation influences a variety of autoimmune disease processes, including Crohn's disease (CD), but the biological function of miRNAs in CD remains unclear. We examine miRNA level in colon tissues and study the potential functions of miRNAs that regulate pathological genes during the inflammation process. Design miRNA levels were assayed in the inflamed colon of 2,4,6-trinitrobenzene sulfonic acid (TNBS)induced and IL-10 knockout (KO) chronic colitis mice and CD patients by microarray or qRT-PCR. The influence of differently expressed miR-141 on its putative target genes, CXCL12 beta, and leukocyte migration was investigated in colonic epithelia cells, colitis models and CD patients. The role of miR-141 was further studied in the experimental colitis mice by intracolonic administration of miR-141 precursors or inhibitors. Results An inverse correlation between miR-141 and CXCL12 beta/total-CXCL12 was observed predominantly in the epithelial cells of the inflamed colons from colitic mice and CD patients. Further study demonstrated that miR-141 directly regulated CXCL12 beta expression and CXCL12 beta-mediated leukocyte migration. Upregulation or downregulation of miR-141 in the TNBS-induced or IL-10 KO colitic colon regulated leukocyte infiltration and alleviated or aggravated experimental colitis, respectively. Additionally, colonic overexpression of CXCL12 beta abolished the therapeutic effect of miR-141 in TNBS-induced colitis. Conclusions This study showed that the pathway of miR-141 targeting CXCL12 beta is a possible mechanism underlying inflammatory cell trafficking during colonic inflammation process. Inhibiting colonic CXCL12 beta expression and blocking colonic immune cell recruitment by using miRNA precursors represents a promising approach that may be valuable for CD treatment.
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