期刊
KIDNEY & BLOOD PRESSURE RESEARCH
卷 40, 期 1, 页码 52-65出版社
KARGER
DOI: 10.1159/000368482
关键词
Connexin; Gap junction; Renal interlobar artery; Vascular smooth muscle cell; Spontaneously hypertensive rats; Vasoconstriction
资金
- National Basic Research Program of China [2012CB52660000]
- National Natural Science Foundation of China [31260247, 31100829, 81000411]
Background/Aims: This study was designed to investigate the expression and function of gap junction protein connexin 45 (Cx45) in renal interlobar artery (RIA) of spontaneously hypertensive rats (SHR), and the association between hypertension and enhanced vasoconstrictive response in SHR. Methods: Western blot analysis and pressure myography were used to examine the differences in expression and function of Cx45 in vascular smooth muscle cells (VSMCs) of RIA between SHR and normotensive Wistar-Kyoto (WKY) rats. Results: Our results demonstrated that 1) whole-cell patch clamp measurements showed that the membrane capacitance and conductance of in-situ RIA VSMCs of SHR were significantly greater than those of WKY rats (p<0.05, n=6), suggesting that the coupling of gap junction between VSMCs of RIA was enhanced in SHR; 2) the KCl or phenylephrine (PE)-stimulated RIA constriction was more pronounced in SHR than that in WKY rats (p<0.05, n=10). After applying a gap junction inhibitor 18 beta-glycyrrhetintic acid (18 beta-GA), the inhibitory effect of 18 beta-GA on KCl or PE-induced vasoconstriction was greater in SHR (p<0.05, n=10); and 3) the expression of Cx45 in RIA of SHR was greater than that in WKY rats (p<0.05, n=3) at 4, 12 and 48 wks of age. Conclusions: The hypertension-induced elevation of Cx45 may affect communication between VSMCs and coupling between VSMCs and endothelium, which results in an increased vasoconstrictive response in renal artery and might contribute to the development of hypertension. Copyright (C) 2015 S. Karger AG, Basel
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