4.2 Article

Glycomimicry: display of fucosylation on the lipo-oligosaccharide of recombinant Escherichia coli K12

期刊

GLYCOCONJUGATE JOURNAL
卷 28, 期 1, 页码 39-47

出版社

SPRINGER
DOI: 10.1007/s10719-010-9322-1

关键词

Bacterial glycosylation; Glycoengineering; Fucosylation; Lewis Y; Chimeric LOS

资金

  1. SNF [31003A_127098/1]
  2. European Community [MEST-CT-2004-5033]

向作者/读者索取更多资源

We recently described the design of Escherichia coli K12 and Salmonella enterica sv Typhimurium to display the gangliomannoside 3 (GM3) antigen on the cell surface [1]. We report here the fucosylation of modified lipooligosaccharide in a recombinant E.coli strain with a truncated lipid A core due to deletion of the core glycosyltransferases genes waaO and waaB. This truncated structure was used as a scaffold to assemble the Lewis Y motif by consequent action of the heterologously expressed beta-1,4 galactosyltransferase LgtE (Neisseria gonorrheae), the beta-1,3 N-acetylglucosaminyltransferase LgtA and the beta-1,3 galactosyltransferase LgtB from Neisseria meningitidis, as well as the alpha-1,2 and alpha-1,3 fucosyltransferases FutC and FutA from Helicobacter pylori. We show the display of the Lewis Y structure by immunological and chemical analysis.

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