期刊
GLYCOCONJUGATE JOURNAL
卷 26, 期 9, 页码 1171-1180出版社
SPRINGER
DOI: 10.1007/s10719-009-9237-x
关键词
Norovirus; Virus-like particle; Glycosphingolipid; ABH antigen; Secretor
资金
- Swedish Research Council [8266]
- Governmental grants (ALF)
- Sahlgrenska University Hospital and the French Region Pays de la Loire (CIMATH)
Noroviruses and norovirus virus-like particles (VLPs) exhibit strain specific patterns in their binding to ABH and Lewis histo-blood group antigens. In this study we demonstrate for the first time specific binding of Norwalk virus VLPs to type 1 and type 2 chain glycosphingolipids (GSLs) carrying ABH and Lewis antigens. N-succinimidyl-3-tributylstannyl benzoate (ATE) was precursor labeled with I-125 and then conjugated to VLPs. The I-125-VLPs were used in GSL thin-layer chromatogram binding assays and displayed binding to H type 1, Lewis b, A type 1, A Lewis b GSLs but no binding to B type 1 or B Lewis b GSLs. For the type 2 chain GSLs the Norwalk VLPs bound to H type 2, Lewis y, A type 2 and A Lewis y. In addition, the VLPs bound to several complex GSLs from blood group O and A, but not from blood group B red blood cells.
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