Developmental regulation of oligosialylation in zebrafish

Zebrafish appears as a relevant model for the functional study of glycoconjugates along vertebrate's development. Indeed, as a prelude to such studies, we have previously identified a vast array of potentially stage-specific glycoconjugates, which structures are reminiscent of glycosylation pathways common to all vertebrates. In the present study, we have focused on the identification and regulation of major protein and lipids associated alpha 2-8-linked oligosialic acids motifs in the early development of zebrafish. By a combination of partial hydrolysis, anion exchange HPLC-FD and mass spectrometry, we demonstrated that glycoproteins and glycolipids differed by the extent and the nature of their substituting oligosialylated sequences. Furthermore, relative quantifications showed that alpha 2-8-linked sialylation was differentially regulated in both families of glycoconjugates along development. Accordingly, we established that alpha 2,8-sialyltransferase mRNA levels was directly correlated with changes of alpha 2,8-sialylation status of glycolipids, but independent of those observed on major glycoproteins that appear to originate from the mother.