期刊
GLYCOBIOLOGY
卷 20, 期 11, 页码 1402-1409出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwq101
关键词
Bifidobacterium; GH2; GH20; lacto-N-neotetraose; prebiotics
资金
- Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN)
Bifidobacteria are predominant in the intestines of breast-fed infants and offer health benefits to the host. Human milk oligosaccharides (HMOs) are considered to be one of the most important growth factors for intestinal bifidobacteria. HMOs contain two major structures of core tetrasaccharide: lacto-N-tetraose (Gal beta 1-3GlcNAc beta 1-3Gal beta 1-4Glc; type 1 chain) and lacto-N-neotetraose (Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc; type 2 chain). We previously identified the unique metabolic pathway for lacto-N-tetraose in Bifidobacterium bifidum. Here, we clarified the degradation pathway for lacto-N-neotetraose in the same bifidobacteria. We cloned one beta-galactosidase (BbgIII) and two beta-N-acetylhexosaminidases (BbhI and BbhII), all of which are extracellular membrane-bound enzymes. The recombinant BbgIII hydrolyzed lacto-N-neotetraose into Gal and lacto-N-triose II, and furthermore the recombinant BbhI, but not BbhII, catalyzed the hydrolysis of lacto-N-triose II to GlcNAc and lactose. Since BbgIII and BbhI were highly specific for lacto-N-neotetraose and lacto- N-triose II, respectively, they may play essential roles in degrading the type 2 oligosaccharides in HMOs.
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