期刊
GLYCOBIOLOGY
卷 21, 期 6, 页码 734-742出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwq192
关键词
bacterial oligosaccharyltransferases; delta-proteobacteria; Desulfovibrionaceae; glycoprotein; protein glycosylation
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Alberta Ingenuity Centre for Carbohydrate Science (AICSS)
- Canada Foundation for Innovation (CFI)
- University of Alberta
- Alberta Ingenuity Fund
- Alberta Heritage Foundation for Medical Research (AHFMR)
- CIHR
Oligosaccharyltransferases (OTases) are responsible for the transfer of carbohydrates from lipid carriers to acceptor proteins and are present in all domains of life. In bacteria, the most studied member of this family is PglB from Campylobacter jejuni (PglB(Cj)). This enzyme is functional in Escherichia coli and, contrary to its eukaryotic counterparts, has the ability to transfer a variety of oligo- and polysaccharides to protein carriers in vivo. Phylogenetic analysis revealed that in the delta proteobacteria Desulfovibrio sp., the PglB homolog is more closely related to eukaryotic and archaeal OTases than to its Campylobacter counterparts. Genetic analysis revealed the presence of a putative operon that might encode all enzymes required for N-glycosylation in Desulfovibrio desulfuricans. D. desulfuricans PglB (PglB(Dd)) was cloned and successfully expressed in E. coli, and its activity was confirmed by transferring the C. jejuni heptasaccharide onto the model protein acceptor AcrA. In contrast to PglB(Cj), which adds two glycan chains to AcrA, a single oligosaccharide was attached to the protein by PglB(Dd). Site-directed mutagenesis of the five putative N-X-S/T glycosylation sites in AcrA and mass spectrometry analysis showed that PglB(Dd) does not recognize the conventional bacterial glycosylation sequon consisting of the sequence D/E-X-1-N-X-2-S/T (where X-1 and X-2 are any amino acid except proline), and instead used a different site for the attachment of the oligosaccharide than PglB(Cj.). Furthermore, PglB(Dd) exhibited relaxed glycan specificity, being able to transfer mono- and polysaccharides to AcrA. Our analysis constitutes the first characterization of an OTase from delta-proteobacteria involved in N-linked protein glycosylation.
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