期刊
GLYCOBIOLOGY
卷 20, 期 11, 页码 1366-1372出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwq102
关键词
N-glycosylation; oligosaccharyltransferase
资金
- ETH Zurich
- Schweizerische Nationalfonds
- Deutsche Forschungsgemeinschaft [DU 1117/1-1]
We describe a phage display technique that allows the production and selective enrichment of phages that display an N-glycoprotein (glycophages). We applied glycophage display to select functional glycosylation sequons from a pool of randomized acceptor sequences. Our system provides a genetic platform to study and engineer different steps in the pathway of bacterial N-linked protein glycosylation.
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