期刊
GLYCOBIOLOGY
卷 20, 期 4, 页码 433-441出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwp190
关键词
Ascomycota; aureobasidin A; glycosphingolipid; Hirsutella rhossiliensis; neogalatriaosylceramide
资金
- The Japan Society for the Promotion of Science [B 16380063]
- Ministry of Education, Culture, Sports, Science, and Technology, Japan
Hirsutella rhossiliensis, a nematophagous fungus belonging to the Ascomycota, is resistant to aureobasidin A (AbA). In this fungus, the biosynthetic pathway leading to mannosylinositolphosphoceramides, which is inhibited by AbA, was not detected. Instead, this fungus contains neutral complex glycosphingolipids (GSLs) and monoglycosylceramides. Except for monoglycosylceramides, neutral GSLs share a neogala-series core structure, Gal beta 1-6Gal beta 1-Cer. Among the GSLs of H. rhossiliensis, three novel GSLs with terminal Man and Glc residues on the sugar chain were elucidated. We analyzed GSL structure using compositional sugar, fatty acid, and sphingoid analyses, methylation analysis, matrix-assisted laser desorption ionization time-of-flight/mass spectrometry (MALDI-TOF MS), and (1)H nuclear magnetic resonance spectroscopy (NMR). The following structures were determined: Man alpha 1-3Gal beta 1-6Gal beta 1-6Gal beta 1-Cer; Glc alpha 1-2Gal beta 1-6Gal beta 1-6Gal beta 1-Cer; and Man alpha 1-3Gal beta 1-6(Glc alpha 1-4)Gal beta 1-6Gal beta 1-Cer. In the ceramides, the fatty acids were predominantly saturated h24:0-acids and the sphingoids were predominately t18:0- or t18:1-sphingoids. In contrast, the ceramides of Glc beta 1-Cer contained d18:2- and d19:2-sphingoids. These findings indicate the presence of a novel biosynthetic pathway of neogala-series GSLs in fungi.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据