期刊
GLIA
卷 60, 期 6, 页码 875-881出版社
WILEY-BLACKWELL
DOI: 10.1002/glia.22320
关键词
oligodendrocyte; white matter injury; matrix metalloproteinase-9; vascular remodeling; cerebral endothelial cell
资金
- Deane Foundation
- MGH ECOR
- American Heart Association
- National Institutes of Health
- Japan Society for the Promotion of Science
- National Research Foundation of Korea
- World Class University
- Global Research Laboratory
- Creative Research Initiative Program
After stroke and brain injury, cortical gray matter recovery involves mechanisms of neurovascular matrix remodeling. In white matter, however, the mechanisms of recovery remain unclear. In this study, we demonstrate that oligodendrocytes secrete matrix metalloproteinase-9 (MMP-9), which accelerates the angiogenic response after white matter injury. In primary oligodendrocyte cultures, treatment with the proinflammatory cytokine interleukin-1 beta (IL-1 beta) induced an upregulation and secretion of MMP-9. Conditioned media from IL-1 beta-stimulated oligodendrocytes significantly amplified matrigel tube formation in brain endothelial cells, indicating that MMP-9 from oligodendrocytes can promote angiogenesis in vitro. Next, we asked whether similar signals and substrates operate after white matter injury in vivo. Focal white matter injury and demyelination was induced in mice via stereotactic injection of lysophosphatidylcholine into corpus callosum. Western blot analysis showed that IL-1 beta expression was increased in damaged white matter. Immunostaining demonstrated MMP-9 signals in myelin-associated oligodendrocytic basic protein-positive oligodendrocytes. Treatment with an IL-1 beta-neutralizing antibody suppressed the MMP-9 response in oligodendrocytes. Finally, we confirmed that the broad spectrum MMP inhibitor GM6001 inhibited angiogenesis around the injury area in this white matter injury model. In gray matter, a neurovascular niche promotes cortical recovery after brain injury. Our study suggests that an analogous oligovascular niche may mediate recovery in white matter. (c) 2012 Wiley Periodicals, Inc.
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