4.6 Article

Intracerebral Granulocyte-Macrophage Colony-Stimulating Factor Induces Functionally Competent Dendritic Cells in the Mouse Brain

期刊

GLIA
卷 57, 期 12, 页码 1341-1350

出版社

WILEY-LISS
DOI: 10.1002/glia.20853

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granulocyte-macrophage colony-stimulating factor; dendritic cells; CNS

资金

  1. Deutsche Forschungsgemeinschaft (DFG) [GRK 320, Ja 690/5-2]

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Granulocyte-macrophage colony-stimulating factor (GMCSF) is a hematopoietic growth factor and a proinflammatory cytokine. While GM-CSF is lacking in normal brain tissue, it is expressed under pathological conditions and correlates with the presence of dendritic cells (DC). However, the role of GM-CSF for the onset of immune responses in the brain is still unclear. To analyze the role of GM-CSF for the induction and functional activity of immune cells in the brain, we performed chronic intracerebroventricular administration of GM-CSF to the brains of adult mice. After GM-CSF administration, intracerebral leukocytes (ICL) were characterized by means of flow cytometry, immunohistochemistry, and an ex vivo functional assay. GM-CSF treatment significantly increased the number of leukocytes expressing high levels of CD45, indicative of peripheral, blood-derived cells. The infiltrating cells were preferentially DC of the myeloid lineage (CD45(high) CD11c(+) CD11b(+)) with an activated phenotype characterized by upregulated expression of MHCII and the costimulatory ligand CD80. Furthermore, DC from GM-CSF treated mice were fully competent to activate naive allogeneic T cells in a mixed leukocyte reaction. In contrast, intracerebroventricular IFN-gamma administration stimulated MHCII expression on cells resembling resident microglia, but did not induce comparable presence of DC. Taken together, intracerebroventricular GM-CSF treatment results in high numbers of DC in the brain. Moreover, these GM-CSF-induced DC display an activated phenotype and exhibit the capacity to act as fully competent DC even without a further inflammatory stimulus. (C) 2009 Wiley-Liss, Inc.

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