期刊
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
卷 16, 期 10, 页码 845-856出版社
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1400319
关键词
Invertase inhibitor; Fruit development; Transient transformation system; Solanum lycopersicum; Overexpression
类别
资金
- National Natural Science Foundation of China [31372054, 30971999]
- State Key Laboratory of Plant Physiology and Biochemistry Open Project of China [SKLPPBKF1404]
In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.
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