期刊
GERIATRICS & GERONTOLOGY INTERNATIONAL
卷 10, 期 -, 页码 S10-S24出版社
WILEY
DOI: 10.1111/j.1447-0594.2010.00601.x
关键词
aging; brain; imaging; ischemia-reperfusion; reactive oxygen
资金
- Japanese Ministry of Education, Culture, Sports, Science and Technology [13470193, 15390366]
- Grants-in-Aid for Scientific Research [13470193, 15390366] Funding Source: KAKEN
Since Harman proposed the free-radical theory of aging, oxidative stress has been postulated to be a major causal factor of senescence. The accumulation of oxidative stress-induced oxidatively modified macromolecules, including protein, DNA and lipid, were found in tissues during the aging process; however, it is not necessarily clear which factor is more critical, an increase in endogenous reactive oxygen and/or a decrease in anti-oxidative defense, to the age-related increase in oxidative damage. To clarify the increasing production of reactive oxygen with age, we examined reactive oxygen-dependent chemiluminescent (CL) signals in ex vivo brain slices prepared from different-aged animal brains during hypoxia-reoxygenation treatment using a novel photonic imaging method. The CL signal was intensified during reoxygenation. The signals in SAMP10 (short-life strain) and SAMR1 (control) brain slices increased with aging. The slope of the increase of CL intensity with age in P10 was steeper than in R1. Age-dependent increase of CL intensity was also observed in C57BL/6 mice, Wistar rats and pigeons; however, superoxide dismutase (SOD) activity in the brain did not change with age. These results suggest that reactive oxygen production itself increased with aging. The rate of age-related increases of CL intensity was inversely related to the maximum lifespan of animals. We speculate that reactive oxygen might be a signaling molecule and its levels in tissue might determine the aging process and lifespan. Decelerating age-related increases of reactive oxygen production are expected to be a potent strategy for anti-aging interventions. Geriatr Gerontol Int 2010; 10 (Suppl. 1): S10-S24.
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