期刊
JOURNAL OF WOOD SCIENCE
卷 61, 期 6, 页码 608-619出版社
SPRINGER JAPAN KK
DOI: 10.1007/s10086-015-1513-8
关键词
Enzymatic dehydrogenative polymerization; Lignification; Monolignol; Dimer; Reaction kinetics
资金
- Grants-in-Aid for Scientific Research [15H01230, 25252032] Funding Source: KAKEN
The structure and biosynthesis of lignin are not yet fully understood, especially the step following the initial dimerization of monolignol. Liquid chromatograph mass spectrometer (LC-MS) was used to analyze the consumption rates of monolignol dimers formed by beta-O-4, beta-5, and beta-beta couplings between coniferyl alcohols in efforts to understand the activity of monolignol dimers in enzymatic dehydrogenative polymerization. We investigated the reaction kinetics in single-component and mixed-component reaction systems containing one and two species of the dimers, respectively. A difference was observed between the consumption rates of the three dimers we tested, and the consumption rate of one dimer in the single-component reaction was different from that in a mixed-component reaction. In qualitative LC-MS analyses, coniferyl alcohol oligomers were detected in the reaction products. Some monolignol tetramers were formed by 5-5 and 5-O-4 coupling between the dimers. The results of this work suggested that monolignol dimers with beta-5 and beta-beta linkages could function as radical mediators in enzyme-catalyzed polymerization.
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