期刊
GEOMICROBIOLOGY JOURNAL
卷 29, 期 3, 页码 255-263出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/01490451.2011.598604
关键词
uranium deposits; microbial community; bacteria; archaea; PCR; DGGE; RFLP; cultivation
资金
- National Basic Research Program of China (973 program) [2007CB815601]
- National Natural Science Foundation of China [40772034, 40902097, 31071745]
- Outstanding Youth Fund [21125730]
- Natural Science Foundation of Fujian Province [2010J06006]
- Key Laboratory of Solid Waste Treatment and Resource Recycle [09zxgk05]
- Ministry of Education
- Chinese Academy of Sciences [KJCX1.YW.07, KJCX2-YW-N50]
- Specialized Research Fund for the Doctoral Program of Higher Education [20093515110010]
- National High Technology Research and Development Program (863 Program) of China [2011AA10A203]
Investigation of microbial communities in uranium deposits will be beneficial for understanding the indigenous microbial impacts on uranium-mineralization as well as developing appropriate remediation and long-term management strategies of uranium-contaminated repositories. In this study, microbial communities of Shihongtan uranium deposits, Xinjiang, China, were investigated using molecular biological techniques and traditional cultivation. PCR-DGGE and PCR-RFLP analyses suggested that there were a number of bacteria and archaea in Shihongtan uranium deposits. Phylogenetic analysis indicated that the bacterial communities were affiliated with Firmicutes, gamma-Proteobacteria and Actinobacteria, while most of archaea showed close evolutionary relationship with Halobacteriaceae. Furthermore, a total of 27 bacterial strains were isolated from 8 core samples, and these strains were most closely related to Clostridium sp., Exiguobacterium sp., Enterobacter sp., Pseudomonas sp., Klebsiella sp., Aeromonas sp, Citrobacter sp., Tessaracoccus sp. and Jonesia sp., respectively. No archaea were cultured. We hope the current investigation of microbial communities in uranium deposits combining culture-dependent and independent approaches, could not only help in understanding the microbial impacts on uranium-mineralization, but also help in identifying microbes that participate in uranium biomineralization for further study.
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