期刊
JOURNAL OF VIROLOGY
卷 89, 期 14, 页码 7428-7432出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00426-15
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资金
- funds from German Cancer Aid [110410]
- European Commission [FP7-HEALTH-2010261506/AIPGENE]
- UK MRC grant [1001764]
- MRC [G1001764] Funding Source: UKRI
- Medical Research Council [G1001764] Funding Source: researchfish
High-throughput integration site (IS) analysis of wild-type adeno-associated virus type 2 (wtAAV2) in human dermal fibroblasts (HDFs) and HeLa cells revealed that juxtaposition of a Rep binding site (RBS) and terminal resolution site (trs)-like motif leads to a 4-fold-increased probability of wtAAV integration. Electrophoretic mobility shift assays (EMSAs) confirmed binding of Rep to off-target RBSs. For the first time, we show Rep protein off-target nicking activity, highlighting the importance of the nicking substrate for Rep-mediated integration.
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