期刊
GENOMICS
卷 93, 期 3, 页码 282-288出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2008.11.005
关键词
Apple; Single nucleotide polymorphisms; Physical map; Genetic map; Bin mapping
资金
- USDA Cooperative State Research, Education and Extension Service - National Research Initiative-Plant Genome Program [2005-35300-15538]
- Illinois Council for Food and Agricultural Research [IDA CF 06FS-0303]
- New Zealand Foundation for Research Science and Technology (FRST) [C06XO207]
A genome-wide BAC physical map of the apple, Malus x domestica Borkh., has been recently developed. Here, we report on integrating the physical and genetic maps of the apple using a SNP-based approach in conjunction with bin mapping. Briefly, BAC clones located at ends of BAC contigs were selected, and sequenced at both ends. The BAC end sequences (BESs) were used to identify candidate SNPs. Subsequently, these candidate SNPs were genetically mapped using a bin mapping strategy for the purpose of mapping the physical onto the genetic map. Using this approach, 52 (23%) out of 228 BESs tested were successfully exploited to develop SNPs. These SNPs anchored 51 contigs, spanning similar to 37 Mb in cumulative physical length, onto 14 linkage groups. The reliability of the integration of the physical and genetic maps using this SNP-based strategy is described, and the results confirm the feasibility of this approach to construct an integrated physical and genetic maps for apple. (C) 2008 Elsevier Inc. All rights reserved.
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