期刊
GENOMICS
卷 94, 期 2, 页码 110-116出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2009.04.004
关键词
Transcription; Intrinsic termination; GeSTer; Eubacteria; Palindrome; Stem-loop; Rho
资金
- Indo-Swiss joint Research Programme of Department of Science and Technology, Government of India
In Escherichia coli, the canonical intrinsic terminator of transcription includes a palindrome followed by a U-trail on the transcript. The apparent underrepresentation of such terminators in eubacterial genomes led us to develop a rapid and accurate algorithm, GeSTer, to predict putative intrinsic terminators. Now, we have analyzed 378 genome sequences with an improved version of GeSTer. Our results indicate that the canonical E. coli type terminators are not overwhelmingly abundant in eubacteria. The atypical structures, having stem-loop structures but lacking 'U' trail, occur downstream of genes in all the analyzed genomes but different phyla show conserved preference for different types of terminators. This propensity correlates with genomic GC content and presence of the factor, Rho. 60-70% of identified terminators in all the genomes show optimized stem-length and Delta G. These results provide evidence that eubacteria extensively rely on the mechanism of intrinsic termination, with a considerable divergence in their structure, positioning and prevalence. The software and detailed results for individual genomes are freely available on request. (C) 2009 Elsevier Inc. All rights reserved.
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