期刊
GENOMICS
卷 94, 期 2, 页码 138-145出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2009.04.003
关键词
Suppression subtractive hybridization; BmNPV; Bornbyx mori; Antiviral response
资金
- Ministry of Science and Technology of China [2006AA10A119]
- National Natural Science Foundation of China [30570074]
- Natural Science Foundation of Zhejiang Province, China [Y307049]
- Specialized Research Fund for the Doctoral Program of Higher Education [20070335102]
We investigated variations in the gene expression of Bombyx mori following infection with a nucleopolyhedrovirus (BmNPV). Two B. mori strains, KN and 306, which are highly resistant and susceptible to BmNPV infection, respectively, were used in this Study. The infection profiles of BmNPV in the B. mori KN and 306 larvae revealed that the virus invaded the midguts of both these strains. However, its proliferation was notably inhibited in the midgut of the resistant strain. By using the suppression subtractive hybridization method, two cDNA libraries were constructed in order to compare the BmNPV responsive gene expressions between the two silkworm lines. In total, 62 differentially expressed genes were obtained. Real-time qPCR analysis confirmed that eight genes were significantly up-regulated in the midgut of the M strain following BmNPV infection. Our results imply that these up-regulated genes may be involved in the B. mori immune response against BmNPV infection. (C) 2009 Elsevier Inc. All rights reserved.
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