期刊
GENOMICS
卷 93, 期 6, 页码 551-564出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2009.01.007
关键词
Leishmania infantum; Shotgun DNA microarrays; Peanut lectin agglutination; Metacyclic promastigotes; Infectivity
资金
- Ministerio de Educacion y Ciencia (MEC) [AGL2003-06152-C02-01]
- Centro de Astrobiologia
- MEC [I3P-BPD2003-1]
- Ministerio de Ciencia y Tecnologia [BMC 2003-00088]
- Comunidad Autonoma de Madrid [GR/SAL/06512004]
Metacyclic promastigotes are transmitted during bloodmeals after development inside the gut of the sandfly vector. The isolation from axenic Cultures of procyclic and metacyclic promastigotes by peanut lectin agglutination followed by differential centrifugation is controversial in Leishmania infantum. The purpose of this study has been to isolate both fractions simultaneously from the same population in stationary phase of axenic culture and compare their expression profiles by whole-genome shotgun DNA microarrays. The 317 genes found with meaningful values of stage-specific regulation demonstrate that negative selection of metacyclic promastigotes by PNA agglutination is feasible in L. infantum and both fractions can be isolated. This subpopulation up-regulates a cysteine peptidase A and several genes involved in lipophosphoglycan, proteophosphoglycan and glycoprotein biosynthesis, all related with infectivity. In fact, we have confirmed the increased infection rate of PNA(-) promastigotes by U937 human cell line infection experiments. These data support that metacyclic promastigotes are related with infectivity and the lack of agglutination with PNA is a phenotypic marker for this subpopulation. (C) 2009 Elsevier Inc. All rights reserved.
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