期刊
GENOMICS
卷 94, 期 3, 页码 196-203出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2009.05.007
关键词
Thellungiella halophila; SALT OVERLY SENSITIVE1; Salt tolerance; Comparative sequence analysis; Microcolinearity; Simple sequence repeats
资金
- Bud Antle Endowed Chair
- United States Department of Agriculture CSREES/Southwest Consortium on Plant Genetics and Water Resources [06NO8/07RO8]
- Department of Energy [DE-FG02-04ER15616]
To provide a framework for studies to understand the contribution of SALT OVERLY SENSITIVE1 (SOS1) to salt tolerance in Thellungiella halophila, we sequenced and annotated a 193-kb T halophila BAC containing a putative SOS1 locus (ThSOS1) and compared the sequence to the orthologous 146-kb region of the genome of its salt-sensitive relative, Arabidopsis thaliana. Overall, the two sequences were colinear, but three major expansion/contraction regions in T halophila were found to contain five Long Terminal Repeat retro-transposons, MuDR DNA transposons and intergenic sequences that contribute to the 47.8-kb size variation in this region of the genome. Twenty-seven genes were annotated in the T halophila BAC including the putative ThSOS1 locus. ThSOS1 shares gene structure and sequence with A. thaliana SOS1 including 11 predicted transmembrane domains and a cyclic nucleotide-binding domain; however, different patterns of Simple Sequence Repeats were found within a 540-bp region upstream of SOS1 in the two species. (c) 2009 Elsevier Inc. All rights reserved.
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