期刊
GENOME RESEARCH
卷 22, 期 6, 页码 1139-1143出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gr.136242.111
关键词
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资金
- NSF
- Lowell Milken Prostate Cancer Foundation
We have adapted transposase-based in vitro shotgun library construction (tagmentation) for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng of input DNA, and ample useful sequences from 1 ng of input DNA. We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to similar to 8.6x high-quality coverage of each strand.
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