期刊
GENOME RESEARCH
卷 21, 期 9, 页码 1506-1511出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gr.121715.111
关键词
-
资金
- National Human Genome Research Institute
- Canadian Institutes of Health Research
- NIH
- SysCODE Consortium (NIH)
- Fondation Leducq
- United States-Israel Binational Science Foundation Jerusalem, Israel [2009290]
- Ragon Institute
- NHLBI
Second-generation sequencing is gradually becoming the method of choice for miRNA detection and expression profiling. Given the relatively small number of miRNAs and improvements in DNA sequencing technology, studying miRNA expression profiles of multiple samples in a single flow cell lane becomes feasible. Multiplexing strategies require marking each miRNA library with a DNA barcode. Here we report that barcodes introduced through adapter ligation confer significant bias on miRNA expression profiles. This bias is much higher than the expected Poisson noise and masks significant expression differences between miRNA libraries. This bias can be eliminated by adding barcodes during PCR amplification of libraries. The accuracy of miRNA expression measurement in multiplexed experiments becomes a function of sample number.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据