期刊
GENOME BIOLOGY AND EVOLUTION
卷 6, 期 10, 页码 2731-2740出版社
OXFORD UNIV PRESS
DOI: 10.1093/gbe/evu216
关键词
unicellular algae; heat resistance; HSP; microarray; environmental adaptation
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Core Research for Evolutional Science and Technology (CREST) of Program of Japan Science and Technology Agency (JST)
- Funding Program for Next Generation World-Leading Researchers (NEXT Program) [GS015]
- [26-786]
- Grants-in-Aid for Scientific Research [14J00786, 26650111] Funding Source: KAKEN
The primitive red alga Cyanidioschyzon merolae inhabits acidic hot springs and shows robust resistance to heat shock treatments up to 63 A degrees C. Microarray analysis was performed to identify the key genes underlying the high temperature tolerance of this organism. Among the upregulated genes that were identified, we focused on two small heat shock proteins (sHSPs) that belong to a unique class of HSP families. These two genes are located side by side in an inverted repeat orientation on the same chromosome and share a promoter. These two genes were simultaneously and rapidly upregulated in response to heat shock treatment (> 1,000-fold more than the control). Interestingly, upregulation appeared to be triggered not by a difference in temperatures, but rather by the absolute temperature. Similar sHSP structural genes have been reported in the green alga Chlamydomonas reinhardtii, but the threshold temperature for the expression of these sHSP-encoding genes in Ch. reinhardtii was different from the threshold temperature for the expression of the sHSP genes from Cy. merolae. These results indicate the possible importance of an absolute temperature sensing system in the evolution and tolerance of high-temperature conditions among unicellular microalgae.
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