期刊
GENOME BIOLOGY AND EVOLUTION
卷 6, 期 2, 页码 290-305出版社
OXFORD UNIV PRESS
DOI: 10.1093/gbe/evu013
关键词
origin of eukaryotes; LECA; Holozoa; eukaryote evolution; chitin synthase; Smad
资金
- Biotechnology and Biological Sciences Research Council [BBSRC - BB/G00885X/2]
- European Research Council [ERC-2007-StG-206883]
- Ministerio de Economia y Competitividad (MINECO) [BFU2011-23434]
- Gordon and Betty Moore Foundation [GBMF3307]
- National Environment Research Council
- MINECO
- BBSRC [BB/G00885X/2, BB/G00885X/1] Funding Source: UKRI
- ICREA Funding Source: Custom
- Biotechnology and Biological Sciences Research Council [BB/G00885X/1, BB/G00885X/2] Funding Source: researchfish
Myosins are key components of the eukaryotic cytoskeleton, providing motility for a broad diversity of cargoes. Therefore, understanding the origin and evolutionary history of myosin classes is crucial to address the evolution of eukaryote cell biology. Here, we revise the classification of myosins using an updated taxon sampling that includes newly or recently sequenced genomes and transcriptomes from key taxa. We performed a survey of eukaryotic genomes and phylogenetic analyses of the myosin gene family, reconstructing the myosin toolkit at different key nodes in the eukaryotic tree of life. We also identified the phylogenetic distribution of myosin diversity in terms of number of genes, associated protein domains and number of classes in each taxa. Our analyses show that new classes (i.e., paralogs) and domain architectures were continuously generated throughout eukaryote evolution, with a significant expansion of myosin abundance and domain architectural diversity at the stem of Holozoa, predating the origin of animal multicellularity. Indeed, single-celled holozoans have the most complex myosin complement among eukaryotes, with paralogs of most myosins previously considered animal specific. We recover a dynamic evolutionary history, with several lineage-specific expansions (e.g., the myosin III-like gene family diversification in choanoflagellates), convergence in protein domain architectures (e.g., fungal and animal chitin synthase myosins), and important secondary losses. Overall, our evolutionary scheme demonstrates that the ancestral eukaryote likely had a complex myosin repertoire that included six genes with different protein domain architectures. Finally, we provide an integrative and robust classification, useful for future genomic and functional studies on this crucial eukaryotic gene family.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据