期刊
GENOME
卷 55, 期 4, 页码 327-335出版社
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
DOI: 10.1139/G2012-014
关键词
crested wheatgrass; F-2 population; AFLP; RAPD; genetic linkage map
资金
- National Basic Research Program of China (973 program) [2007CB108901]
- National Natural Science Foundation of China [31172254]
- Ministry of Education [20091515-110002]
- national supported planning project of science and technology [2008BADB 3B03]
Using a population of 105 interspecific F-2 hybrids derived from a cross between Agropyron mongolicum Keng and Agropyron cristatum (L.) Gaertn. 'Fairway' as a mapping population, a genetic linkage map of crested wheatgrass was constructed based on AFLP and RAPD molecular markers. A total of 175 markers, including 152 AFLP and 23 RAPD markers, were ordered in seven linkage groups. The map distance was 416 cM, with a mean distance of 2.47 cM between markers. The number of markers ranged from 13 to 46 in each linkage group and the length of groups ranged from 18 to 104 cM. The research found that 30 out of 175 molecular markers showed segregation distortion, accounting for 17% of all markers. This is the first genetic linkage map of crested wheatgrass. This map will facilitate gene localization, cloning, and molecular marker-assisted selection in the future.
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