期刊
GENOME
卷 53, 期 9, 页码 688-697出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/G10-056
关键词
Bombyx mori; gamma-aminobutyric acid receptor; GABAR gene family; RDL subunit; evolution
资金
- National Science Foundation of China [30828003, 30770280]
- National Basic Research Program of China [2010CB126205]
- National Program of High-Tech Research and Development [2006AA10A119]
gamma-Aminobutyric acid (GABA) is a very important inhibitory neurotransmitter in both vertebrate and invertebrate nervous systems. GABA receptors (GABARs) are known to be the molecular targets of a class of insecticides. Members of the GABAR gene family of the silkworm, Bombyx mori, a model insect of Lepidoptera, have been identified and characterized in this study. All putative silkworm GABAR cDNAs were cloned using the reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Bombyx mori appears to have the largest insect GABAR gene family known to date, including three RDL, one LCCH3, and one GRD subunit. The silkworm RDL1 gene has RNA-editing sites, and the RDL1 and RDL3 genes possess alternative splicing. These mRNA modifications enhance the diversity of the silkworm's GABAR gene family. In addition, truncated transcripts were found for the RDL1 and LCCH3 genes. In particular, the three RDL subunits may have arisen from two duplication events.
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