期刊
GENETICS AND MOLECULAR RESEARCH
卷 12, 期 3, 页码 2913-2922出版社
FUNPEC-EDITORA
DOI: 10.4238/2013.August.12.7
关键词
Taxol-producing fungus; Protoplast; Genetic transformation; Agrobacterium tumefaciens
资金
- China National 863 High-Tech Program
- Shanghai Science and Technology Committee
An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304' AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly investigated and optimized. PCR amplification and Southern hybridization were used to verify the transformation events. This study should pave the way for future genetic modification studies of Ozonium sp EFY21.
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