期刊
GENETICS AND MOLECULAR RESEARCH
卷 12, 期 2, 页码 1269-1277出版社
FUNPEC-EDITORA
DOI: 10.4238/2013.April.17.5
关键词
Rice; Proline; Osmotic stress; DNA methylation
资金
- Jilin Provincial Science & Technology Department [201205048]
- National Natural Science Foundation of China [30970232, 30870178]
- Fundamental Research Funds for the Central Universities [11QNJJ020]
Proline accumulation is involved in plant osmotic stress tolerance. Given that DNA methylation is related to almost all metabolic processes through regulation of gene expression, we suspected that this epigenetic modification and proline biosynthesis are probably related. To test this, we investigated whether osmotic stress-induced proline accumulation is associated with DNA methylation modifications in rice. We assessed DNA methylation and expression of 3 key genes (P5CR, P5CS, and delta-OAT) involved in proline biosynthesis, and measured proline content under both osmotic stress (15% polyethylene glycol) and control conditions. After osmotic stress, selfed progenies of osmotic-stressed plants accumulated higher concentrations of proline in leaves under both normal conditions and under osmotic stress than the unstressed control plants. Concomitantly, under osmotic stress, the selfed progeny plants showed higher expression levels of P5CS and d-OAT than the control. This up-regulated expression was stably inherited by the subsequent generation. Methylation-sensitive Southern blotting indicated that 2 of the 3 genes, P5CS and delta-OAT, had greater DNA demethylation in the selfed progenies than in the control. Apparently DNA demethylation facilitated proline accumulation by up-regulating expression of the P5CS and delta-OAT genes in response to osmotic stress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据