期刊
JOURNAL OF VIROLOGICAL METHODS
卷 211, 期 -, 页码 32-35出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2014.10.006
关键词
Rice stripe virus; Rice black-streaked dwarf virus; Simultaneous detection via RT-PCR
资金
- National Natural Science Foundation of China [31170142]
- Jiangsu Agricultural Scientific Self-innovation Fund [CX[14]5019]
- Special Fund for Agro-scientific Research in the Public Interest of China [201003031]
Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV) are transmitted by their common vector small brown planthopper (SBPH) that cause serious crop losses in China. A simple reverse transcription-PCR method was developed for the simultaneous detection of RSV and RBSDV in single SBPH. Three primers targeted to RSV-RNA4 and RBSDV-S2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. The method is reliable, rapid and inexpensive for detecting the two viruses in vector, which could facilitate better forecasting and control of the virus diseases. Using this method, it was found that SBPH could carry RSV and RBSDV simultaneously. (C) 2014 Elsevier B.V. All rights reserved.
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