期刊
GENETICS AND MOLECULAR RESEARCH
卷 11, 期 1, 页码 434-439出版社
FUNPEC-EDITORA
DOI: 10.4238/2012.February.24.2
关键词
Apostichopus japonicus; Microsatellite; Polymorphic marker
资金
- National Key Technology R&D Program of China [2011BAD13B05, 2011BAD13B06]
- National High Technology Research and Development Program of China [2012AA10A412]
The sea cucumber (Apostichopus japonicus) is an important item in Asian cuisine. It is currently produced through aquaculture, especially in China, after being overexploited in the wild in the 1990s. We isolated 70 novel polymorphic microsatellite loci using an enrichment-colony hybridization protocol. All loci were characterized in 48 individuals from a natural population in Rongcheng (Shandong, China) using genomic DNA isolated from muscle tissue. The number of alleles ranged from 2 to 17 (mean 7.0), and the observed and expected heterozygosities varied from 0.0010 to 1.0000 and from 0.2125 to 0.9477, respectively. Thirty-one of the 70 loci exhibited departure from Hardy-Weinberg equilibrium. These microsatellite markers should be useful resources for population genetic studies and for molecular marker-assisted breeding of A. japonicus.
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