期刊
JOURNAL OF VIROLOGICAL METHODS
卷 223, 期 -, 页码 55-60出版社
ELSEVIER
DOI: 10.1016/j.jviromet.2015.07.014
关键词
Tobamovirus; Cucumber; Melon; Pollen grains; Anther; CGMMV
资金
- Chief Scientist Foundation of the Ministry of Agriculture of Israel - The Israeli CGMMV Initiative [132-1740]
Cucumber green mottle mosaic virus (CGMMV), which belongs to the genus Tobamovirus, is a major pathogen of cucurbit crops grown indoors and in open fields. Currently, immunology (e.g., ELISA) and molecular amplification techniques (e.g., RT-PCR) are employed extensively for virus detection in plant tissues and commercial seed lots diagnostics. In this study, a fluorescent in situ hybridization (FISH) technique, using oligonucleotides whose 5'-terminals were labeled with red cyanine 3 (Cy3) or green fluorescein isothiocyanate (FITC), was developed for the visualization of the pathogen in situ. This simple and reliable method allows detection and localization of CGMMV in the vegetative and reproductive tissues of cucumber and melon. When this technique was applied in male flowers, anther tissues were found to be infected; whereas the pollen grains were found to be virus-free. These results have meaningful epidemiological implications for the management of CGMMV, particularly with regard to virus transfer via seed and the role of insects as CGMMV vectors. (C) 2015 Elsevier B.V. All rights reserved.
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