期刊
GENETICS AND MOLECULAR BIOLOGY
卷 34, 期 4, 页码 640-U217出版社
SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572011000400017
关键词
khat; (S)-cathinone; phenylpropylamino alkaloids biosynthesis; EST library; gene discovery
资金
- Binational Agricultural Research and Development Fund [CA-9117-09]
- Israel Science Foundation [814/06]
Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to >98% of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified.
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